2014   Program   Parallel Sessions
SUNDAY, 18 MAY, 2014
10:45 - 12:15

PARALLEL SESSION 1
Image Cytometry

A novel excitation-scanning hyperspectral microscope for lung tissue imaging
Minimizing stains and maximizing throughput for cell cycle and mitotic phase identification using imaging flow cytometry  
Detection and enumeration of circulating tumor cells using Imaging Flow Cytometry   
Hyper-dimensional analysis for label-free high-throughput imaging flow cytometry

PARALLEL SESSION 2
Standards and Calibration

Evaluation of statistical photoelectron (Spe) scales using quadratic fitting of LED series data and of multilevel bead set data to facilitate comparisons of instrument sensitivity   
Comparison of instrument sensitivity (Q) and background (B) for 10 dyes of interest across an array of different flow cytometers
A method to compare antibody-labeled iPSC colonies from day to day and across laboratories
Man Versus Machine: Statistical Validation and Efficiency of Pipelined Analysis of a Clinical Flow Cytometry Study      

PARALLEL SESSION 3
SRL Management

Quality system and certification iso 9001-2008: more benefits than disadvantages for cell cytometry facility.    
A benchmarking analysis of flow core facilities within the Cambridge Cytometry Network
Lessons Learned from Optimization of in vitro Diagnostic Cell Based Flow Cytometric Assays.
Flow Cytometry in Clinical Trials: Global Method Validation  

PARALEL SESSION 4
Immunology

Using Imaging Flow Cytometry to study the spatio-temporal pattern of CD3-Induced Calcium Flux in T cells.   
Immunopathological viral encephalitis: mobilisation of monocyte progenitors and infiltration into the CNS
Fluorescence lifetime imaging reveals NADPH oxidase overactivation as a mechanism of multiple sclerosis pathogenesis
Eleven-color Flow Cytometry and 5-way Cell Sorting for accurate measurement of T cell subsets, their differentiation, cytotoxicity, apoptosis and T-cell receptor diversity: characterizing the immune response in human cutaneous Leishmaniasis.  

PARALLEL SESSION 5
Novel Diagnostic Tools and Method Optimization

mRNA Assessment of BCR & ABL using DNA Branching Technique and subsequent detection with Flow Cytometry
High Throughput Multiplexed Assays to Facilitate Detection of Shiga Toxin Producing E. Coli in the Beef Chain
A multiplex-able image cytometry platform for breast cancer immunophenotyping via fluorescence and surface enhanced Raman scattering (SERS) spectral imaging
A novel assay for rapid diagnosis of myocardial infarction by lectin-mediated red blood cell agglutination 

MONDAY, 19 MAY, 2014
10:15 - 11:45

PARALLEL SESSION 6
Flow Cytometry Instrumentation

Spectral flow cytometry of fluorescent proteins
Influence of Laser Attributes on Submicron Particle Detection and Resolution
Research and Clinical Applications of Autofluorescence as a Cytoparameter
Radiofrequency encoded angular-resolved light scattering for rapid, high-resolution, label-free particle classification  

PARALLEL SESSION 7
Flow Cytometry Data and Analysis I

FlowCAP-III: Results from Community Evaluation of Methods for Automated Analysis of Flow Cytometry  Data 
ASPIRE: Anomalous Sample Phenotype Identification under Random Effects. A case study of Hierarchical Dirichlet Processes use in cytometry data analysis.
CyTOFLOCK: Density-Based Subspace Data Clustering for Identification and Comparison of Cell Populations in Mass Cytometry Data  
Cell population mapping across flow cytometry samples using the FlowMap-FR algorithm and its application to identify cellular biomarkers of an allergic response

PARALEL SESSION 8
Cell Proliferation & Death

Non-genotoxic stabilization of p53 in human osteosarcoma cells promotes transition to tetraploidy via mitotic bypass 
In-depth characterisation of drug effects on cell cycle using Chipcytometry
Apoptotic volume decrease measured with scanning flow cytometry
Screening for fingerprint patterns of cell cycle routing in mesenchymal progenitor and osteosarcoma populations  

PARALEL SESSION 9
Measuring Immune Responses

Cancer exome analysis reveals neo-antigen-specific T cell reactivity in melanoma patients  
Fine tuning of Treg and iNKT cells after treatment with Fingolimod in Multiple Sclerosis patients
Increased sensitivity of positivity assessment for intracellular cytokine staining across multiple HVTN clinical trials using the Bayesian MIMOSA method
Counting cytokine-producing T helper cells: Concentration of calcium in the medium can be critical

PARALLEL SESSION 10
Regenerative Medicine

Impact of low oxygen level on human adipose-derived stem cells isolated from lean and obese donors
Native Extracelluar Matrix: A New Scaffolding Platform for Regenerative Medicine
Histologic location of CD90+ epithelial cells and tissue macrophages in normal and malignant esophagus
Surface protein expression on adipose-derived stromal cells (ASC) and bone marrow-derived mesenchymal stromal cells  

TUESDAY, 20 MAY, 2014
10:15 - 11:45

PARALLEL SESSION 11
New Probes and Assays

Cancer Stem Cell Identification and Sorting: Advancing Current Technologies & Methods   
New self-checking fluorogen-exchange assays for GPCR desensitization and desensitization
Live cell sorting of heterogeneous populations based on RNA and protein detection  
A novel FRET multiplex flow cytometry assay for concomitant detection of actin, tubulin and vimentin filaments dynamics  

PARALLEL SESSION 12
Flow Cytometry Data Analysis II

Automatic Gating Strategy Identification for Polychromatic and Mass Flow Cytometry Data
flowMatch: Template-based Classification and Hierarchical Organization of Flow Cytometry Data Collections Hierarchical Organization of Flow Cytometry Data Collections 
An entropy-based computational approach for guiding gating strategy in clustering high-dimensional flow data.  
Characterizing antigen-specific T-cells through the computational integration of flow cytometry and gene expression data at the single-cell level  

PARALLEL SESSION 13
Immune Monitoring

Leukocyte subsets correlate with gender, age and lifestyle factors: multicolor flow cytometric analysis for the LIFE study      
Human naive-derived T memory stem cells are preferentially responsible for post-transplant T cell reconstitution.  
Principal Component Analysis allows to cluster patients with Multiple Sclerosis on the basis of different subsets of CD8+ and iNKT cells
Age Independent Trends in Subpopulations of CD8+ T-Cells from Cancer Patients Undergoing Chemotherapy

PARALLEL SESSION 14
New Instruments

Sorting Cells Based Upon Fluorescence Lifetime Changes In FRET  
An Advanced Prototype Instrument for Clinical Cell Sorting
Surface Acoustic Wave (SAW) Based 'Smart' Cell Sorters
Versatile printed disposable chambers with on-chip sample preparation for simple and affordable point-of-care cell counting     

PARALLEL SESSION 15
Optical Imaging and Analysis

Mitochondrial Morphology and Membrane Potential Quantification via Automated High Content Image Analysis  
Cytometry of Cells in Motion: Multiparameter Analysis in Dark Field  
Subcellular Cytometry - Quantitative 3-D Analysis of Discrete Subcellular Structures and Events
Large-scale time-Lapse Microscopy of the dynamics of OCT-4 Expression in Human Embryonic Stem Cell Cultures

WEDNESDAY, 21 MAY, 2014
10:15 - 11:45

PARALLEL SESSION 16
Cell Sorting and Selection

Single live cell  analysis and sorting using mRNA and miRNA markers
Enabling Single-Cell Gene Expression Analysis in Rare Events Combining FACS and the C1 Fluidigm System
Implementation of a fast and efficient method to sort rare cells
A silicon microfluidic-based platform for high-speed lensfree imaging analysis and sorting of cells  

PARALLEL SESSION 17
Flow Cytometry Data Analysis III

Standardizing Flow Cytometry Immunophenotyping: Automated Gating Recapitulates Central Manual Analysis with Low Variability  
ISAC's Gating-ML 2.0: Is analysis exchange between software tools finally becoming a reality?
Cell population identification using Fluorescence-Minus-One controls with a one-class classifying algorithm
CytoGenius: software that addresses all of flow cytometry's primary information challenges

PARALLEL SESSION 18
Personalized Medicine

Peripheral monocytes as biosensors to monitor type I interferon reponses in viral infection and autoimmunity
Multi-color, multi-parameter high-throughput flow cytometry for compound screening using correlated cell physiology signatures  
Reliable Measurement of Proportions and Absolute Cell Counts from Fixed and Cryopreserved Whole Blood
Prediction of Clinical Response to Anti-TNF Therapy for Inflamatory Bowel Diseases  by Circulating T Cell Subsets but not by B Cells

PARALLEL SESSION 19
Analysis of Micro- and Sub-Micron Particles

An Imporved Flow Cytometry Strategy to Quantify Extracellular Vesicles from Plasma
Nanoparticle flow cytometry of individual extracellular vesicles​
Small Particle Analysis By Flow Cytometry
OutsideIn: Analysis of nanoparticle history at the cellular level  

PARALLEL SESSION 20
High Throughput Instrumentation

High Throughput Digital Video Flow Cytometry
Suspension arrays coded by tunable luminescence lifetimes
Acoustically Enhanced High Throughput Flow Cytometry for Remote Plankton Monitoring  
Development of a fully automated ultra-high throughput flow cytometry screening system to enable novel drug discovery  
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