2015 Program Workshops

SATURDAY, JUNE 27
14:30 - 16:00

WORKSHOP 1
Lost in Translation
Virginia Litwin,Covance Central Laboratory Services, USA
Steven Eck, MedImmune, USA
Cherie Green, Genentech, USA
Wendy White, MedImmune, USA

Fiona Germaschewski, GlaxoSmithKline, United Kingdom

The ability of flow cytometry to characterize large numbers of suspended cells by simultaneously assessing multiple markers with a reasonable degree of quantitation makes the technology very attractive for use in clinical trials.  The utilization of flow cytometry in clinical drug development generally falls into a few broad categories including assays that help determine subject enrollment, evaluate safety, test hypotheses, or drive hypothesis generation.  Enrollment determining assays are intended to establish whether an individual meets the predefined study specifications and are classified as either safety or biomarker assays.  Safety assays typically examine a known or suspected health risk, the presence of which could impact the risk/benefit assessment of the drug or warrant clinical intervention for the study subject.  Hypothesis testing assays generally have a pre-specified analysis sufficient to test a specific hypothesis and typically include pharmacodynamics (PD) and biomarker assays.  The last category tend to be more purely discovery endeavors looking to identify a potential biomarker and/or PD markers associated with response to treatment or mechanism of action and typically involve tissue (most commonly blood) profiling to look for a pattern of response. Examples of the different types of assays and considerations around their implementation in translational studies will be discussed.

WORKSHOP 2
Cytometry with Tunable Ultrafast Lasers?
Toralf Kaiser, Flow Cytometry and Cellsorting Facility, Germany
Jens Bethge, APE Angewandte Physik und Elektronik GmbH, Germany
Konrad von Volkmann, APE Angewandte Physik und Elektronik GmbH, Germany
Raluca Niesner, DRFZ, Germany
David Reismann, DRFZ Germany
Bill Telford, National Cancer Institute, USA


Recently, the reliability of ultra fast light sources has been greatly improved. However, in imaging cytometry and in flow cytometry these light sources are currently seeing only limited use, although first experiments with ultrafast lasers have shown promising results. Here we report on the latest generation of light sources extending the tuning range of classical lasers and providing multiple wavelength output. An overview of their use in advanced microscopy methods such as fluorescence lifetime based methods, CARS, and SRS is presented. Based on the reported methods an open discussion will be held with an expert panel and the audience to investigate the challenges and advantages of cytometry with ultrafast lasers.

WORKSHOP 3
Metrics of a Successful SRL: Publications and Acknowledgements
Derek Davies, Cancer Research UK, United Kingdom
Andy Filby, London Research Institute, Cancer Research UK, United Kingdom


This interactive workshop will focus on the ways that a Shared Resource Laboratory can use publications as a metric of the success of a facility but will also look at the ways that we can be pro-active. This pro-activity may be as simple as ensuring that users properly acknowledge work that is carried out in a service Laboratory. However, we can also set up collaborations with users at either our Institutional settings or externally. Or we may be able to carry out independent research. There are barriers to all of these so the aim of this workshop is to look at ways in which these can be overcome and ideally settle upon 'best practice' guidelines.

WORKSHOP 4
Microvesicle Workshop
Philip Hexley, University of Nebraska Medical Center, USA
Joanne Lannigan, University of Virginia, USA

In recent years the cytometric analysis of microvesicles has increased dramatically, however there still remains much controversy in the literature due in part to lacking standardization and calibration. This workshop will focus on key foundations aimed to move the field forward. These are, guidelines for reviewers and editors in manuscript submission of cytometric microvesicles, and the current status of standardization and calibration.

16:30 - 18:00

WORKSHOP 5
When Worlds Collide: Research Complexity and Clinical Validity of Flow Cytometric Assays used for Drug Development
Virginia Litwin,Covance Central Laboratory Services, USA
Thomas McCloskey, ICON Labs, USA
David Lanham, Eurofins, United Kingdom
Christele Gonneau, Covance, Switzerland
Maxime Moulard, France


Clinical flow cytometric methods used guide patient diagnosis and treatment decisions must be validated according to CAP/CLIA requirements.  The innovative, high complexity methods used to support basic research do not require formal validation.  The application of high complexity methods during the drug development process presents several challenges surrounding the validation approach.  This workshop will address the numerous factors which must be considered when developing a validation study plan using the Fit-for-Purpose approach and challenges of implementing novel technology in a regulated environment. 

WORKSHOP 6
Mucosal Immunology
Nancy Fisher, University of North Carolina at Chapel Hill, USA
Calum Bain, MRC Centre for Inflammation Research, United Kingdom

This workshop will highlight some of the advances in understanding Mucosal Immunity with novel cytometry approaches.

Abstract:  Innate Immunity serves as the front line of defense from invading pathogens in the mucosal tissues. With our growing understanding of the mucosal tissues, so is our realization of the complexity of the cell types and processes. Multi-parameter flow cytometry has influenced our understanding of these complex sites by allowing separation and visualization of cellular lineages, origins, plasticity and trafficking. We encourage discussion about new methods for tracking and visualizing innate immune cells in the mucosal tissues. 

Agenda:

  • Introduction: Working with Primary Cells from the Mucosa. Nancy C. Fisher, Chapel Hill, USA
  • Unravelling the Mononuclear Phagocyte Compartment of the Gust Mucosa. Calum Bain, Edinburgh, Scotland
  • Polychromatics Flow Cytometry in Cancer Stem Cells Characterization from Lung Cancer Samples. Alicia Martinez-Romero, Valencia, Spain. Abstract 378
  • Using Multispectral Imaging Flow Cytometry to Assess Ricin Trafficking in Human Lung Epithelial-Like A549 Cells. Dominic Jenner, Salisbury, UK. Abstract 349
  • Effect of Soluble ST2 on Activation of Type 2 Innate Lymphoid Cells in Murine Model of Asthma. Hiroko Hayakawa, Tochigi, Japan. Abstract 168.

WORKSHOP 7
Management: Dealing with Difficult Situations
Rui Gardner, Instituto Gulbenkian de Ciencia, Protugal
Joanne Lannigan, University of Virginia, USA


An interactive workshop where you will learn how to identify, approach, resolve, manage and prevent those difficult situations which are often encountered in the day to day management of a Shared Resource Facility. Bring your smartphone!

WORKSHOP 8
Direct Discovery of Disease Mechanisms in Human Tissue and Options for Efficient Therapeutic Intervention using Imaging Cycler Microscopy: A New Clinically Validated Paradigm
Walter Schubert, University of Magdeburg, Germany
Shan-e-Ahmed Raza, University of Warwick, United Kingdom


Learning about and discuss a microscopic technique capable of decoding the spatial disease mechanisms directly in tissues and derive valid therapeutic principle.
 
The presentations will comprise three parts:
 
1. Schubert W. Technology: Three-dimensional functional super-resolution and in situ order of biomolecular systems
 
2. Shan. S. Bioinformatics pipeline for the analysis of multiplexed image data.

  • Round table discussion including short contributions: All participants
3. Schubert W. Clinical Validation: Correct therapy prediction in ALS and direct in-tissue decoding of unexpected disease mechanisms.
  • Round table discussion: what is semantic decoding of diseased tissues? How to use it for systematic decoding of the human toponome? All participants

MONDAY, JUNE 29
16:00 - 17:30

WORKSHOP 9
Flow Cytometry Trends and Drivers
Giacomo Vacca, Kinetic River Corp, USA

Innovation is everywhere, but out of many new concepts only a few emerge that have staying power. What distinguishes them? While the success of a new technology can be the result of complex factors, the most effective progress in a field is often made when powerful new technologies fill critical unmet market needs. With such a match, adoption spreads, and change is rapid. In this workshop, a panel of speakers will first discuss several key current trends in cytometry, the market drivers behind those trends, and the enabling technologies poised to make a difference. The interactive discussion to follow will give the audience the opportunity to add diverse perspectives to the mix.

WORKSHOP 10
The Devil is in the Details: Flow Cytometry Methods in Global Multicenter Trials
Virginia Litwin, Covance Central Laboratory Services, USA
Nick Jones, LabCorp, USA
Alessandra Vitaliti, Switzerland
Lisa Patti-Diaz, USA

Cellular biomarkers (pharmacodynamic, mechanistic, safety, efficacy) have been proven to be very valuable in early clinical studies.  This workshop will address the challenges and opportunities associated with the implementation of sophisticated, customized and standardized flow cytometry-based biomarker assays in multi-center clinical studies.  Topics to be addressed include: ex vivo stimulation, sample handling at the sites, training of sites; stabilization, freezing, shipment of samples; within study controls; outsourcing of flow cytometry assays.

WORKSHOP 11
Multilaser Cytometry: Developments, Challenges, and Pitfalls
Adrian Smith, Centenary Institute, Australia
Bill Telford, National Cancer Institute, USA


While much of the focus on expanding flow cytometry capabilities has been aimed at maximising the number of parameters (the “emission” side of the technology), a concurrent line of technology development has also been occurring on the excitation side as well. This workshop will focus on the excitation side, namely the optimisation of the laser systems that drive emission from fluorescent reagents. Laser technology has advanced significantly in the past ten to fifteen years and it is now possible to purchase lasers of almost any wavelength and outfit flow cytometers with up to ten different laser lines. This flexibility provides great opportunities for optimising multiparametric experiments but it comes at the cost of increased complexity and potential resultant confusion. In this workshop we will attempt to address that complexity through presentations and discussion, in particular, we will look at (i) current laser technologies and options to consider when purchasing or upgrading instruments, (ii) the advantages and disadvantages of different wavelengths in the context of different experimental requirements, (iii) laser power optimisation, (iv) potential pitfalls and cautions in multi-laser cytometry, (v) new trends and emerging laser technologies (including an update on tunable lasers) and (vi) the identification of areas where further testing and experimentation is required.

WORKSHOP 12
Educating Users in New Advancements in Cytometry
Matt Cochran, University of Rochester Medical Center, USA
Michele Black,University of Washington, USA


This workshop explores ways Shared Resource Laboratories can provide educational opportunities to their users on advancements in cytometry. One of the challenges in cytometry is staying informed of rapidly evolving technologies and reagents. As an SRL, providing services to researchers, it is vital that the lab keeps current and can act as a conduit for information to the researchers with advancements that can directly impact their work.  The aim of the workshop is to find ways an SRL can stay up to date on technology and provide ideas on how best to disseminate information to the users.

WORKSHOP 13
Back to the Future: CYTOMICS - Translational System Cytometry
Raif Yuecel, University of Aberdeen, United Kingdom
Paul Smith, Cardiff University, United Kingdom
Atilla Tarnok, University of Leipzig, Germany


Towards better understanding of Cellular Systems we need to study the single cell in the heterogeneity of cytomes by applying the new generation of methodologies in Flow and Imaging Cytometry.

This “CYTOMICS – Workshop” will provide an overview of the next generation of technologies to study the cells in their physiological environment in vivo, within the system of an organism: “In vivo Flow Cytometry” and “In vivo Imaging”.
We believe this CYTOMICS workshop will be a platform within the Cytometry Society to discuss cutting-edge technologies that bridges the gap of the “Translational Cytometry from bench to bedside”.  Let us greet old friends and make new ones in this platform.

 

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