33rd Congress of the International Society for Advancement of Cytometry
   
First Time Attendee & New Member Orientation
Keynote Lectures
Invited Speakers
Scientific Tutorials
Workshops
Commercial Tutorials
CYTO Innovation
CYTO Youth
Social Activities
Mobile App
Program at a Glance
SRL Education & Networking Event
Sponsors

Sunday, April 29
1230-1330

Thermo Fisher Scientific
102 Fountain Crescent
Inchinnan Business Park
Paisley, United Kingdom, PA4 9RE
Email:  jennifer.buick@thermofisher.com
Web:  www.thermofisher.com

Room: South Hall 2A
Presenter:  Oggie Golub, PhD
Research and Development Scientist
Thermo Fisher Scientific
Title: Essential tools for image-based cytometry using fluorescent labeling and detection methods

The combination of light microscopy and fluorescent reporters offers an unparalleled view into the structure and function of intact cells. Thermo Fisher Scientific instruments and reagents have been at the cutting edge of fluorescent reporter development for over four decades. In this seminar, we will review our portfolio of long-proven tools and protocols that have enabled researchers to confidently create publication quality cell images on the first attempt. Whether you’re new to cell imaging, or an experienced researcher wanting to use your imaging platform at its fullest potential, join us to learn about the latest innovations in fluorescence microscopy. Topics include an overview of automated image acquisition and cytometry using a spinning disk confocal High Content Analysis (HCA) platform, as well as imaging reagents that reduce background fluorescence, amplify fluorescent signal output, prevent photobleaching, and improve spatial resolution of 3D samples. 
 

ZELLKRAFTWERK

Deutscher Platz 5c
Leipzig, Germany 04103
Email: detmers@zellkraftwerk.com
Web:  www.zellkraftwerk.com
 
Room: North Hall
Presenter:  Christian Henning

Title: Update on ChipCytometry: One platform, many applications.
Over the last 2 years, ChipCytometry has been increasingly used as an emerging platform by over 30 labs around the world for different purposes. Zellkraftwerk has now added more functionality and applications to this platform and would like to introduce you to the most recent updates and applications:
  1. TrueBiobanking: With ChipCytometry, you can store and ship cells and tissues under controlled conditions for at least 24 month without loss of biomarkers or shift in population composition. Furthermore, analysis is destruction free, samples can be re-analyzed for additional biomarkers any time later after primary analysis rendering this method extremely useful for precious samples. This is particular useful for patient samples in transcontinental, multicenter clinical trials. We will give you an update on handling and biobanking procedures as well as on quality data on storage stability and validation of data quality of clinical trial assays set up with ChipCytometry.
  2. MassiveMultiplexing: Currently, Chipcytometry, is the only platform enabling to combine 100 markers on one sample, since we use serial 5plex assays to overcome limitations of too many colors on too many antibodies at the same time. We will update you on data about latest massive multiplexing assays set up with ChipCytometry.
  3. TissueCytometry:With ChipCytometry, you can easily do massive multiplexing on tissue sections, too. We and our customers have generated data on many different tissue types ranging from placenta, liver, kidney, skin to salivary glands and complete mouse brain sections. During this workshop we want to demonstrate both, beauty and usefulness of data obtained by TissueCytometry - also using virtual reality data visualization.
  4. VideoCytometry: With VideoCytometry, we recently added an additional application that allows you to activate and stimulate living cells on the chip surface whilst monitoring biomarker expression changes over time following thousands of cells on the single cell level.
 In brief the tutorial will give a broad overview on new ChipCytometry applications and present case studies from the Early Access Program. We will introduce you to our modular products ranging from Zellscanner ONE to CYTOBOT automation platform. Innovators will be invited to participate in the broadened Early Access Program 2018.
Food will be provided to 100 attendees on a first come first serve basis.
 

Merck

Boulevard Industrial Park, Padge Road
Beeston, Nottingham United Kingdom, NG9 2JR
Email:  nicola.douglas@external.merckgroup.com
​Web:  www.merckmillipore.com

Room: South Hall 2B
Speakers: Thomas Montague, Christine Probst, Vidya Venkatachalam, Robert Smith-McCollum, Merck KGaA, Darmstadt, Germany
Title:
 Introducing the CellStream® System, A Highly-Sensitive, 7-Laser Flow Cytometer
Today Merck launches the new CellStream® benchtop flow cytometry system, a highly-customizable and compact flow cytometer that is the first to use a camera for detection. Its unique optics system and design provides researchers with unparalleled sensitivity and flexibility when analyzing cells and submicron particles.  Within the CellStream® system, the Amnis® time delay integration (TDI) and camera technology rapidly captures low resolution cell images and transforms them into high-throughput intensity data. Researchers acquire the intensity data they are accustomed to from traditional flow cytometers, but with greater fluorescence sensitivity. Join us in this workshop to take a deeper look inside the instrument design, system performance and software of this exciting new system.

 

Monday, April 30

​1230-1330


FlowJo, LLC
385 Williamson Way
Ashland, OR 97520
Phone: 541-201-0022
Email: caitlinf@flowjo.com
Web: www.flowjo.com

Room: Terrace 2A
Presenter:
Mike Stadnisky, Ph.D. 
Title: Single Cell Alchemy: Transformation, Creation, & Combination​

Alchemy was the medieval forerunner of chemistry which aimed to combine base metals and achieve transmutation into gold.  Practiced very actively in Prague in the 16th century, we return four centuries later to the theme of combinations producing something greater than the sum of its parts:

(1) statistical rigor + discovery tools can combine for a new paradigm in single cell analysis

(2) limitless discovery enabled by protein + gene expression measured on the same cells with sequence-tagged antibodies

(3) the potential unlocked by FlowJo + BD.


Miltenyi Biotec GmbH
Friedrich-Ebert-Straße 68
Bergisch-Gladbach, Germany 51429
Phone:  +49-2204-830630
Email: beatel@miltenyibiotec.de
Web:  www.miltenyibiotec.com/en.aspx
 
Room: South Hall 2A
Presenter
 1: Chris Groves, Senior Manager Respiratory Research, MedImmune LLC, Gaithersburg, USA
Title: The automated shared resource … welcoming our new “robot overlords”
 
Presenter 2:
Stefan Radtke Ph.D., Division of Clinical Research, Fred Hutchinson Cancer Research Center, Seattle, USA
Title: Closed-system sorting for stem cell-mediated HIV gene therapy
With flow cytometrists continuing to revolutionize the applications and the utility of analytic and sorting technologies, the focus of this commercial tutorial is to highlight future trends in flow cytometry. The tutorial explores two growing segments which include full application automation from robotic integration and sorting of materials that require closed processing loops. We believe these two topics represent two of the many growing trends in the flow cytometry market.

Sony Biotechnology Inc.
1730 North First St. 
San Jose, CA 95112
Phone:  800-275-5963
Email: sales@sonybiotechnology.com
Web:  www.sonybiotechnology.com
 
Room: North Hall
Presenter: Deena Soni
Title: Precision without complexity – modern multi-application sorting with a two-step approach

Sony presents key capabilities of its automated microfluidics cell sorter. Multicolor flexibility and software versatility adapting to applications will be showcased.
Advanced automation for optical alignment, sort setup and system maintence will be discussed.

BD Biosciences
2350 Qume Drive
San Jose, CA 95131
Phone: 408-954-6026
Email: carrie.carruthers@bd.com
Web: www.bdbiosciences.com

Room: South Hall I
Presenter: Robert Balderas, VP Biological Sciences, BD Biosciences​
Title: Technological Advancements in FACS

In the short history of FACS, the continuous development of new technologies has allowed thousands of discoveries and a deeper understanding of the underlying function of cells from a variety of tissue sources. From the first commercial cell sorter by BD, the complexity of instruments has moved from 1 to 2 colors to 30 and above, linking the contributions of receptor biology, the increased numbers of fluorophores/dyes and the engineering in electronics, optics, fluidics, software, bioinformatics and other integral domain subassemblies. The foundational principle has never changed: as an instrument that provides isolation of individual cells from a heterogeneous population of cells. The result of all of this is the recovery and isolation of a cell maintaining functional integrity for downstream analysis including expansion (cell therapy), gene and protein expression analysis. Today we discuss seminal advancements in high parameter cell analysis and sorting. Included in this presentation will be a history of technological advancements enabling the engineering of integral domain subassemblies and representative data.
 
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Beckman Coulter Life Science
22, rue Juste-Olivier
Nyon, Switzerland 1260 
Email: ukoenig@beckman.com
Web: www.beckman.com
 
Room: Forum Hall
Presenter:
Dr. med. James Hutchinson
Department of Surgery, University Hospital Regensburg
Title: Knowing the Future: A Guide to Predicting Clinical Outcomes
Clinicians take daily decisions about treatment of patients without knowing the precise outcome in each case. In these circumstances, predictive tests to estimate an individual’s likelihood of a therapeutic or adverse effect could be very valuable. Advances in standardized clinical flow cytometry have opened the possibility of collecting complex datasets that capture information about a patients’ immunological status before, during and after therapy (Streitz et al. Transplant Res., 2013). Extracting outcome-associated parameters from immune monitoring data in order to build predictive models can be challenging, especially when contending with real-world limitations of clinically heterogeneous populations and restricted sample sizes. In this commercial tutorial, we explore how clinical insight can shape the design, conduct and interpretation of studies aimed at developing predictive tests. Specifically, we take the example of establishing an assay using DuraClone IM Panels (Beckman Coulter) to predict early viral control under direct-acting antiviral therapy for chronic hepatitis C virus infection (Hutchinson et al. Frontiers in Immunology, 2018). This study illustrates the value of defining clear, medically important questions in designing efficient clinical studies, but also emphasizes the utility of repeated sampling from patients under therapy.

Bio-Rad Laboratories (CYTO)
2000 Alfred Nobel Drive
Hercules, CA 94547
Phone: 510-408-2158
Email: joanna_megdadi@bio-rad.com
Web: www.bio-rad.com

Room: Panorama Hall
Presenter:
Dr. Andrea Cossarizza, ISAC President-Elect and Professor of Pathology and Immunology, University of Modena and Reggio Emilia School of Medicine
Title: Exploring the Potential of the Immune System in Cancer and Infections
Combining the approaches of cellular and molecular technologies can provide new information that is crucial not only for a better understanding of the functionality of the immune system, but also for helping clinicians who are providing advanced and expensive therapies to patients affected by different pathologies.

During this presentation, Dr. Cossarizza will discuss new data and publications from his own and other groups that use cell sorting and Droplet Digital™ PCR. Topics will include:

• The advanced use of flow cytometry in recognizing cell populations that change during severe sepsis and viral infections

• Molecular and cellular changes in the immune system that occur in cancer patients treated with new drugs in the category of immune checkpoint inhibitors

• How new therapeutic strategies for HIV infection can be monitored

Fluidigm Corporation
7000 Shoreline Ct. #100
South San Francisco, CA 94080
Phone: 650-452-0638
Email: connie.cruz@fluidigm.com
Web: www.fluidigm.com

Room: South Hall 2B
Presenters:
JSusanne Heck, PhD
Head of Flow Cytometry Research Platform
NIHR Biomedical Research Centre
Guy's and St Thomas' NHS Foundation Trust/King’s College London
 
Jared Burks, PhD
Assistant Professor
Co-Director, Flow Cytometry and Cellular Imaging Core Facility
Department of Leukemia
MD Anderson Cancer Center

Title: Establishing Imaging Mass Cytometry in a core facility to empower highly-multiplexed tissue imaging
Many flow cytometry cores offer highly-multiplexed mass cytometry services using Helios™, a CyTOF® system. Drs. Heck and Burks were among the first of a growing number of core lab leaders who are expanding their mass cytometry service offerings to include Imaging Mass Cytometry™ (IMC™) services on the Hyperion™ Imaging System. Developed using proven Fluidigm® CyTOF® technology, this system sets a new standard in highly multiplexed tissue imaging by enabling the detection of up to 37 protein markers within the context of the tissue microenvironment. In this workshop, Drs. Heck and Burks will share their perspectives and insights regarding establishing IMC at their facilities. Included will be a brief overview of IMC, requirements to acquire and set up an instrument in a core facility, and discussion of how this system can be integrated into a full core service offering. Information on the workflow and experimental design will be shared, and example data will be presented along with a discussion of data analysis. Drs. Heck and Burks will take questions for the last 15 minutes of the program. 
 

Tuesday, May 1

​1230-1330


ACEA Biosciences Inc.
6779 Mesa Ridge Road, #100
San Diego, CA  92121
Phone:  858-724-0928
Email:  info@aceabio.com
Web:  www.aceabio.com

Room: Terrace 2A
Presenter: Garret Guenther
Title: New High Performance Flow Cytometer - Enable. Disrupt. Science.
The NovoCyte Quanteon provides capabilities that further exceed the requirements for high-end, multi-color panels and advanced experiments with multi-excitation options and flexible signal collection options. Scientists with all levels of flow cytometry experience are afforded ultimate flexibility to choose from 25 fluorescence channels, utilizing four lasers. Exchangeable filters are automatically detected when changed so the desired configuration can suit different needs. It has enhanced automation capabilities to efficiently handle multi-format samples including FACS tubes, 24-,48-,  96-, and 384-well plates plus bar code reading. The excellent NovoExpress® software user experience is maintained with the new addition of many features to improve your analysis capabilities. 

BD Biosciences
2350 Qume Drive
San Jose, CA 95131
Phone: 408-954-6026
Email: carrie.carruthers@bd.com
Web: www.bdbiosciences.com
 
Room: South Hall I
Presenter:  Christina Chang, PhD, Senior Scientist, BD Biosciences​
Title: New technologies for multi-omics immune phenotyping in single cells​

High-throughput single-cell RNA sequencing recently emerged as a powerful tool to profile complex cell populations. Current solutions are limited by the ability to capture multi-omics information which make immune phenotyping of cells with low transcript abundance and similar gene expression profiles challenging. New approaches for protein detection enabled by oligo-conjugated antibodies enables simultaneous detection of mRNA and protein in single cells.  The addition of protein expression data to gene expression data results in more robust clustering of single-cell data compared to clustering by gene expression alone. Comparison of mRNA and protein expression revealed distinct expression profiles for many genes, underscoring the intricacies of transcriptional and translational regulatory mechanisms, and the importance of multi-omics analysis as a tool for immune phenotyping.  We will show results from recent studies where a cell-surface antibody panel was created and used for protein profiling alongside gene expression profiling.  We will discuss how our study demonstrates the value of combining protein and gene based expression data to gain a more comprehensive understanding of cell lineage and function at the single-cell level.

Beckman Coulter Life Science
22, rue Juste-Olivier
Nyon, Switzerland 1260 
Email: ukoenig@beckman.com
Web: www.beckman.com
 
Room: Forum Hall
Presenter:
Dr. Alfonso Blanco Fernández 
Scientific Director of Core Technologies 
Director of Flow Cytometry 
UCD - Conway Institute
Title: Label‐free characterization of microvesicles using a combination of scatter and autofluorescence from multiple lasers
Traditional flow cytometry (FCM) has utilized the intrinsic properties of cells to scatter light as a primary characterization tool since the inception of the technology. The 488 nm laser is most commonly used for this purpose, however 405 nm laser light is superior for resolution of microvesicles. Particle analyzers like the NanoSight select the laser based on the nature of the material to be characterized. However, only one laser can be used at a time and the parameters are limited to concentration and size distribution of the particles within the sample.
In FCM, multiple lasers are used consecutively for measuring many parameters in a sample including the intrinsic light scattering properties from the 488 or 405 nm lasers. This concept was applied to various sample types using the range of laser lines on the Beckman Coulter CytoFLEX LX and the BD
FACSAriaIIIu in order to investigate their utility for label‐free characterization.
Microvesicles from cell lines and clinical samples have been tested using this concept. The results demonstrate that the combination of multiple scatter signals can elucidate patterns that correlate to cell treatment or patient progression.
Samples from chemical industries (insecticides) have been analysed with the Beckman Coulter CytoFLEX LX. The data show that using a combination of autofluorescence together with the scatter light from multiple lasers could improve the QC and testing of these products.
The combination of multiples SSC from the different lasers shows a huge potential for the identification and characterization of different types of populations of cells, microvesicles and/or
particles. Since they are label free parameters, the workflow is simplified and the potential for introducing error is minimized.
Manufacturers and researchers have an opportunity to work side by side to explore and expand these possibilities and develop instruments which are clearly expanding the possibilities of conventional FCM.
The CytoFLEX LX is for Research Use Only. The Beckman Coulter product and service marks mentioned herein are trademarks or registered trademarks of Beckman Coulter, Inc. in the United States and other countries. All other trademarks are the property of their respective owners. FLOW‐3554CP03.18

Bio-Rad Laboratories
2000 Alfred Nobel Drive
Hercules, CA  94547
Phone: 510-408-2158
Email: joanna_megdadi@bio-rad.com
Web: www.bio-rad.com

Room: Panorama Hall
Presenter 1
Jillian Blanck, Research Technician II, Cytometry Core Facility, Stowers Institute for Medical Research
Title: High-Throughput Cytometry Using the Bio-Rad ZE5 with Automation for Characterization of Protein Self-Assembly
High-throughput flow cytometers enable new advancements to a previously underutilized screening tool, with many notable benefits over traditional screening methods. Our work is one such example. Using the Bio-Rad ZE5 Cell Analyzer with automation, we are able to acquire data at high speed without losing sensitivity. This fast sample-loading system, combined with automation for running multiple consecutive plates, allows us to run a 96-well plate in 10 minutes, a 384-well plate in 40 minutes. We are using this technology to characterize protein self-assembly in vivo in the yeast Saccharomyces cerevisiae in a high-throughput and yet unprecedentedly quantifiable manner. We use FRET as a proxy to report on protein self-assembly. When proteins lie in close proximity to one another, the donor fluorophore, when excited, will transfer energy (FRET) to the acceptor fluorophore, resulting in its emission. Quantifying FRET enables us to differentiate between dispersed monomers and self-assemblies of proteins of interest. By using a photoconvertible fluorophore (mEos3.1) bound to the protein of interest and expressed in a high-copy 2-micron plasmid with robust selection, we are able to explore a wide range of protein concentrations in a population of cells. Reproducible photoconvertibility of mEOS3.1 ensures reliably uniform ratios of donor and acceptor states across samples and runs. Several yeast prions are now known, and sequence predictions have elucidated an enrichment of such prion-like domains, indicating the need to mine the S. cerevisiae proteome for phase-separating proteins. I will present on the results of this screen and discuss similar ongoing screens, such as those of the effect of systematic single gene deletions and chaperone overexpressions on prion formation and a screen for exploring the promiscuity of interactions between low-complexity sequences from yeast.

Presenter 2Michael Gregory, Technical Director Cytometry and Cell Sorting Laboratory at New York University Langone Health and ISAC SRL Emerging Leader
Title: Implementing New Instrumentation in Shared Resource Laboratories 
As flow cytometry becomes a widely used technique, it is not only the immunologist looking to use flow cytometry Shared Resource Laboratory (SRL) facilities, but scientists in disciplines from all areas of cell biology. The SRL of today has a goal to help users generate data while providing access to the best instruments available, and even more importantly, to introduce new instrument capabilities best suited for their users' needs. Introducing new instruments can be a challenge, especially to a user group accustomed to using an already established instrument type. In acquiring two ZE5 Cell Analyzers our SRL had to rise to meet this challenge, making use of the new capacity the instruments offered our busy SRL, while ensuring users they would now be able to generate the best data possible. To do so, we implemented a program designed to demonstrate new instrument capabilities, compare data quality to currently used instruments, and encourage participation in comprehensive instrument training in addition to getting hands-on experience. The ZE5 Cell Analyzers aided this program greatly as the new and improved features, data quality, user experience and ease of use all contributed to a dramatic rise in instrument use as users responded positively to our efforts to diversify their experience with flow cytometers. 
 
De Novo Software
400 North Brand Blvd. Ste. 850
Glendale, CA 91203
Phone: 213-814-1240-805
Email: chris.concannon@denovosoftware.com
Web: www.denovosoftware.com

Room: North Hall
Presenter: Dr. David Novo

Title: Using FCS Express to Accelerate your Flow Cytometry Reporting and Results

Most flow cytometry data analysis software focuses on generating plots, gates and stats. But those are rarely your final results. Most researchers spend a significant amount of time transferring data from their flow analysis software into other packages (like Excel, Prism, Jump, PowerPoint and many others) for downstream processing. This dramatically increases the time it takes for you to get your results. FCS Express 6 has a wide variety of tools to bring your final results to you directly in your flow analysis software. Integrated bar and pie charts update instantly as you change your gates. A fully functional spreadsheet lets you apply sophisticated calculations and regressions that change automatically with your analysis. Direct R integration as well as SPADE and vSNE plots provide state of the art high dimensional analysis and visualization. New index sorting compatibility allows for quick single cell and well review at a click.  Come see how FCS Express can help you produce your results in record time.
 
As a special bonus, we will be showing a sneak peak of some of the new features coming down the road.
 

Fluidigm Corporation
7000 Shoreline Ct. #100
South San Francisco, CA 94080
Phone: 650-452-0638
Email: connie.cruz@fluidigm.com
Web: www.fluidigm.com

Room: South Hall 2B
Presenters:
Jonathan M. Irish, PhD
Assistant Professor of Cell and Developmental Biology
Scientific Director, Mass Cytometry Center of Excellence
Vanderbilt University
 
Greg Stelzer, PhD
Director of Mass Cytometry Market Development
Fluidigm
Title: Mass Cytometry for Clinical Research: Establishing a Systems Immune Monitoring Program​
In the past decade, the number of immune cell subsets identified as phenotypically and functionally distinct has significantly expanded. Mass cytometry, which enables analysis of more than 40 parameters per cell, is revolutionizing our ability to discover, characterize, and track rare, clinically significant cell populations in samples of human tumors, tissue, and blood. 
In this tutorial, Dr. Irish will share his experience establishing mass cytometry services for the Vanderbilt research community, including the development of immune monitoring panels, as well as streamlined workflows for sample acquisition and computational analysis. He will also show how mass cytometry is being used at Vanderbilt to characterize immunotherapy responses in blood and tumors.
Dr. Stelzer will present an overview of a new product for use on Helios™ mass cytometry systems, the Maxpar® Human Immune Monitoring Panel Kit. In addition to providing reagents and pre‑titrated antibodies that streamline panel optimization and provide reliable cell staining, the kit comes with access to a third-party analysis software solution providing automated population identification and enumeration.

For Research Use Only. Not for use in diagnostic procedures.

Thermo Fisher Scientific

102 Fountain Crescent
Inchinnan Business Park
Paisley, United Kingdom, PA4 9RE
Email:  jennifer.buick@thermofisher.com
Web:  www.thermofisher.com

Room: South Hall 2A
Presenter:  Jordi Petriz, PhD
Group Leader, Functional Cytomics Group
Josep Carreras Leukaemia Research Institute, Barcelona, Spain 

Title: What's NxT and How to Make Visible the Invisible

Acoustic Focusing Cytometers are analyzers that precisely align cells using ultrasonic waves prior to analysis with one or more lasers. The additional use of flat top profiles for the laser beam intensity allows for a better alignment even when super fast acquisition is used. Current implementations of acoustic focusing cytometers are also called as acoustic assisted hydrodynamic focusing cytometers, injecting acoustically pre-focused cells in the sample core stream into a sheath manifold, where they are further focused by sheath fluid. Acoustic focusing of cells in acoustically driven capillaries used for cytometry does not damage cells, making this technology available for rapid analysis of cells as well as to assist us in gaining understanding in the complex biology of the cells, origins, functions and activation states in combination with immunophenotypic markers.
I will discuss how the acoustic focusing technology can be applied to better understand the acoustic orientation effects with data collected over conventional fixed integrated angles of low angle forward and orthogonal side scatter. While many cells and microorganisms have similar scatter profiles contrast under hydrodynamic focusing, the acoustic focusing cytometers provide significantly different scatter signals for nearly identically shaped cells. Moreover, it will be discussed how the unique capabilities of acoustic focusing cytometry in association with 561 nm laser excitation can improve human hematopoietic counting protocols as well as to facilitate future clinical applications with complex multicolor panels, such as those including more than one functional cellular aspect in combination with cell immunophenotyping. I will also discuss how sample manipulation and red blood cell lysis can damage and provoke the loss of specific cells, as well as the benefits of using violet side scatter and no-lyse no-wash protocols for the identification of leukocytes, for rare cell detection in blood samples, as well as for sensitive detection of myeloid derived suppressor cells or small particles in fluids.
 

Wednesday, May 2

​1230-1330


Cytek Biosciences Inc. 
46107 Landing Parkway
Fremont, CA 94538
Phone: 510-657-0102
Email: greinin@cytekbio.com
Web: https://cytekbio.com/

Room: North Hall
Presenter: Dr. Maria Jaimes

Title: Expanding Application Capabilities Using Full Spectrum Cytometry
Flow cytometry has become a well-established tool in the fields of immunology and cell biology, but it presents some challenges. For example, it is challenging using flow for higher complexity panels. Additional lasers, custom filters, specialized optical configurations, and highly complex and expensive cytometers are required. Moreover, a high level of expertise is needed for panel design, and traditional methods for analyzing data from these panels are insufficient. It is also challenging to identify one cytometer that contains the application flexibility required by flow core labs. For example, special options or an application-specific cytometer are required for applications such as small particle detection. Additionally, flow applications such as microRNA prove challenging using a conventional cytometer due to the autofluorescent nature of cells. In this tutorial, we will present how high sensitivity full spectrum technology enables the Aurora cytometer to overcome these challenges and expand the breadth of applications that can be run on the same cytometer. Furthermore, we will demonstrate how the high-quality data produced can be used for principle component analysis of higher complexity panels. 


 
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