33rd Congress of the International Society for Advancement of Cytometry
   
First Time Attendee & New Member Orientation
Keynote Lectures
Invited Speakers
Scientific Tutorials
Workshops
Commercial Tutorials
CYTO Innovation
CYTO Youth
Social Activities
Program at a Glance
Sponsors

Sunday, April 29
1230-1330

Thermo Fisher Scientific
102 Fountain Crescent
Inchinnan Business Park
Paisley, United Kingdom, PA4 9RE
Email:  jennifer.buick@thermofisher.com
Web:  www.thermofisher.com

Room: South Hall 2A
Presenter:  Oggie Golub, PhD
Research and Development Scientist
Thermo Fisher Scientific
Title: Essential tools for image-based cytometry using fluorescent labeling and detection methods

The combination of light microscopy and fluorescent reporters offers an unparalleled view into the structure and function of intact cells. Thermo Fisher Scientific instruments and reagents have been at the cutting edge of fluorescent reporter development for over four decades. In this seminar, we will review our portfolio of long-proven tools and protocols that have enabled researchers to confidently create publication quality cell images on the first attempt. Whether you’re new to cell imaging, or an experienced researcher wanting to use your imaging platform at its fullest potential, join us to learn about the latest innovations in fluorescence microscopy. Topics include an overview of automated image acquisition and cytometry using a spinning disk confocal High Content Analysis (HCA) platform, as well as imaging reagents that reduce background fluorescence, amplify fluorescent signal output, prevent photobleaching, and improve spatial resolution of 3D samples. 
 

ZELLKRAFTWERK

Deutscher Platz 5c
Leipzig, Germany 04103
Email: detmers@zellkraftwerk.com
Web:  www.zellkraftwerk.com
 
Room: North Hall
Presenter:  Christian Henning
Title: 100-plex Image Cytometry Assay​

ChipCytometry is a high-content cytometry platform combining the unsurpassed quantitative phenotyping ability of flow cytometry with the unparalleled information depth of microscopy. Besides some features that are quite similar to conventional flow cytometry, three technology features make ChipCytometry an exciting technology for explorative high-content analysis: 1. Long-term sample storage/ no sample consumption: ChipCytometry uses microfluidic chips enabling biomarker-preserving long-term storage of samples for a period of at least 24 months. Cell storage is particularly useful for precious samples like patient samples, rare cells and (multicenter) clinical trials because cells are not destroyed during transport, storage and analysis and can be stored for further tests exploring more and more markers on these pre-analyzed samples. 2. High-content cytometry: ChipCytometry enables highly multiplexed biomarker assays. Zellkraftwerk presents a 100-plex immune monitoring assay for comprehensive human immune cell phenotyping, and a 39-plex murine immune monitoring panel. 3. Tissue Cytometry: ChipCytometry can work with as sorts of cell specimens as well as with a broad range of tissue types. The tutorial will give an overview on new ChipCytometry applications and present case studies from the Early Access Program. Innovators will be invited to participate in the broadened Early Access Program 2018 Join us for an introduction to the future of “precious samples cytometry”.
 

Merck

Boulevard Industrial Park, Padge Road
Beeston, Nottingham United Kingdom, NG9 2JR
Email:  nicola.douglas@external.merckgroup.com
​Web:  www.merckmillipore.com

Room: South Hall 2B
Speaker: TBD

 

Monday, April 30

​1230-1330


FlowJo, LLC
385 Williamson Way
Ashland, OR 97520
Phone: 541-201-0022
Email: caitlinf@flowjo.com
Web: www.flowjo.com

Room: Terrace 2A
Presenter: TBD


Miltenyi Biotec GmbH
Friedrich-Ebert-Straße 68
Bergisch-Gladbach, Germany 51429
Phone:  +49-2204-830630
Email: beatel@miltenyibiotec.de
Web:  www.miltenyibiotec.com/en.aspx
 
Room: South Hall 2A
Presenter: TBD


Sony Biotechnology Inc.
1730 North First St. 
San Jose, CA 95112
Phone:  800-275-5963
Email: sales@sonybiotechnology.com
Web:  www.sonybiotechnology.com
 
Room: North Hall
Presenter: Deena Soni


BD Biosciences
2350 Qume Drive
San Jose, CA 95131
Phone: 408-954-6026
Email: carrie.carruthers@bd.com
Web: www.bdbiosciences.com

Room: South Hall I
Presenter: Robert Balderas, VP Biological Sciences, BD Biosciences​
Title: Technological Advancements in FACS

In the short history of FACS, the continuous development of new technologies has allowed thousands of discoveries and a deeper understanding of the underlying function of cells from a variety of tissue sources. From the first commercial cell sorter by BD, the complexity of instruments has moved from 1 to 2 colors to 30 and above, linking the contributions of receptor biology, the increased numbers of fluorophores/dyes and the engineering in electronics, optics, fluidics, software, bioinformatics and other integral domain subassemblies. The foundational principle has never changed: as an instrument that provides isolation of individual cells from a heterogeneous population of cells. The result of all of this is the recovery and isolation of a cell maintaining functional integrity for downstream analysis including expansion (cell therapy), gene and protein expression analysis. Today we discuss seminal advancements in high parameter cell analysis and sorting. Included in this presentation will be a history of technological advancements enabling the engineering of integral domain subassemblies and representative data.
 
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Beckman Coulter Life Science
22, rue Juste-Olivier
Nyon, Switzerland 1260 
Email: ukoenig@beckman.com
Web: www.beckman.com
 
Room: Forum Hall
Presenter: TBD


Bio-Rad Laboratories (CYTO)
2000 Alfred Nobel Drive
Hercules, CA 94547
Phone: 510-408-2158
Email: joanna_megdadi@bio-rad.com
Web: www.bio-rad.com

Room: Panorama Hall
Presenter:
Dr. Andrea Cossarizza, ISAC President-Elect and Professor of Pathology and Immunology, University of Modena and Reggio Emilia School of Medicine
Title: Exploring the Potential of the Immune System in Cancer and Infections
Combining the approaches of cellular and molecular technologies can provide new information that is crucial not only for a better understanding of the functionality of the immune system, but also for helping clinicians who are providing advanced and expensive therapies to patients affected by different pathologies.

During this presentation, Dr. Cossarizza will discuss new data and publications from his own and other groups that use cell sorting and Droplet Digital™ PCR. Topics will include:

• The advanced use of flow cytometry in recognizing cell populations that change during severe sepsis and viral infections

• Molecular and cellular changes in the immune system that occur in cancer patients treated with new drugs in the category of immune checkpoint inhibitors

• How new therapeutic strategies for HIV infection can be monitored

Fluidigm Corporation
7000 Shoreline Ct. #100
South San Francisco, CA 94080
Phone: 650-452-0638
Email: connie.cruz@fluidigm.com
Web: www.fluidigm.com

Room: South Hall 2B
Presenter: TBD

 

Tuesday, May 1

​1230-1330


ACEA Biosciences Inc.
6779 Mesa Ridge Road, #100
San Diego, CA  92121
Phone:  858-724-0928
Email:  info@aceabio.com
Web:  www.aceabio.com

Room: Terrace 2A
Presenter: Garret Guenther
Title: Affordable Flow Cytometer Platform that Enable Disrupt Science​
The NovoCyte Quanteon provides capabilities that further exceed the requirements for high-end multi-color panels and advanced experiments with multi-excitation and flexible signal collection options. Scientists of all levels of flow cytometry experience are enabled to perform up to 25-color experiments using four laser systems. The system can be configured with filters that suit different needs. It has enhanced automation capabilities to efficiently handle multi-format samples formats including tubes, 24-, 96-, and 384-well plates plus bar code tracking capabilities. The excellent NovoExpress® software user experience is maintained with new additional statistical analysis modules. 

BD Biosciences
2350 Qume Drive
San Jose, CA 95131
Phone: 408-954-6026
Email: carrie.carruthers@bd.com
Web: www.bdbiosciences.com
 
Room: South Hall I
Presenter:  Christina Chang, PhD, Senior Scientist, BD Biosciences​
Title: New technologies for multi-omics immune phenotyping in single cells​

High-throughput single-cell RNA sequencing recently emerged as a powerful tool to profile complex cell populations. Current solutions are limited by the ability to capture multi-omics information which make immune phenotyping of cells with low transcript abundance and similar gene expression profiles challenging. New approaches for protein detection enabled by oligo-conjugated antibodies enables simultaneous detection of mRNA and protein in single cells.  The addition of protein expression data to gene expression data results in more robust clustering of single-cell data compared to clustering by gene expression alone. Comparison of mRNA and protein expression revealed distinct expression profiles for many genes, underscoring the intricacies of transcriptional and translational regulatory mechanisms, and the importance of multi-omics analysis as a tool for immune phenotyping.  We will show results from recent studies where a cell-surface antibody panel was created and used for protein profiling alongside gene expression profiling.  We will discuss how our study demonstrates the value of combining protein and gene based expression data to gain a more comprehensive understanding of cell lineage and function at the single-cell level.
Presenter: Michael Gregory, Technical Director Cytometry and Cell Sorting Laboratory at New York University Langone Health and ISAC SRL Emerging Leader
Title: Implementing New Instrumentation in Shared Resource Laboratories 
As flow cytometry becomes a widely used technique, it is not only the immunologist looking to use flow cytometry Shared Resource Laboratory (SRL) facilities, but scientists in disciplines from all areas of cell biology. The SRL of today has a goal to help users generate data while providing access to the best instruments available, and even more importantly, to introduce new instrument capabilities best suited for their users' needs. Introducing new instruments can be a challenge, especially to a user group accustomed to using an already established instrument type. In acquiring two ZE5 Cell Analyzers our SRL had to rise to meet this challenge, making use of the new capacity the instruments offered our busy SRL, while ensuring users they would now be able to generate the best data possible. To do so, we implemented a program designed to demonstrate new instrument capabilities, compare data quality to currently used instruments, and encourage participation in comprehensive instrument training in addition to getting hands-on experience. The ZE5 Cell Analyzers aided this program greatly as the new and improved features, data quality, user experience and ease of use all contributed to a dramatic rise in instrument use as users responded positively to our efforts to diversify their experience with flow cytometers. 

Beckman Coulter Life Science
22, rue Juste-Olivier
Nyon, Switzerland 1260 
Email: ukoenig@beckman.com
Web: www.beckman.com
 
Room: Forum Hall
Presenter:
Jillian Blanck, Research Technician II, Cytometry Core Facility, Stowers Institute for Medical Research
Title: High-Throughput Cytometry Using the Bio-Rad ZE5 with Automation for Characterization of Protein Self-Assembly
High-throughput flow cytometers enable new advancements to a previously underutilized screening tool, with many notable benefits over traditional screening methods. Our work is one such example. Using the Bio-Rad ZE5 Cell Analyzer with automation, we are able to acquire data at high speed without losing sensitivity. This fast sample-loading system, combined with automation for running multiple consecutive plates, allows us to run a 96-well plate in 10 minutes, a 384-well plate in 40 minutes. We are using this technology to characterize protein self-assembly in vivo in the yeast Saccharomyces cerevisiae in a high-throughput and yet unprecedentedly quantifiable manner. We use FRET as a proxy to report on protein self-assembly. When proteins lie in close proximity to one another, the donor fluorophore, when excited, will transfer energy (FRET) to the acceptor fluorophore, resulting in its emission. Quantifying FRET enables us to differentiate between dispersed monomers and self-assemblies of proteins of interest. By using a photoconvertible fluorophore (mEos3.1) bound to the protein of interest and expressed in a high-copy 2-micron plasmid with robust selection, we are able to explore a wide range of protein concentrations in a population of cells. Reproducible photoconvertibility of mEOS3.1 ensures reliably uniform ratios of donor and acceptor states across samples and runs. Several yeast prions are now known, and sequence predictions have elucidated an enrichment of such prion-like domains, indicating the need to mine the S. cerevisiae proteome for phase-separating proteins. I will present on the results of this screen and discuss similar ongoing screens, such as those of the effect of systematic single gene deletions and chaperone overexpressions on prion formation and a screen for exploring the promiscuity of interactions between low-complexity sequences from yeast. 


Bio-Rad Laboratories
2000 Alfred Nobel Drive
Hercules, CA  94547
Phone: 510-408-2158
Email: joanna_megdadi@bio-rad.com
Web: www.bio-rad.com

Room: Panorama Hall
Presenter: TBD


De Novo Software
400 North Brand Blvd. Ste. 850
Glendale, CA 91203
Phone: 213-814-1240-805
Email: chris.concannon@denovosoftware.com
Web: www.denovosoftware.com

Room: North Hall
Presenter: Dr. David Novo

Title: Using FCS Express to Accelerate your Flow Cytometry Reporting and Results

Most flow cytometry data analysis software focuses on generating plots, gates and stats. But those are rarely your final results. Most researchers spend a significant amount of time transferring data from their flow analysis software into other packages (like Excel, Prism, Jump, PowerPoint and many others) for downstream processing. This dramatically increases the time it takes for you to get your results. FCS Express 6 has a wide variety of tools to bring your final results to you directly in your flow analysis software. Integrated bar and pie charts update instantly as you change your gates. A fully functional spreadsheet lets you apply sophisticated calculations and regressions that change automatically with your analysis. Direct R integration as well as SPADE and vSNE plots provide state of the art high dimensional analysis and visualization. New index sorting compatibility allows for quick single cell and well review at a click.  Come see how FCS Express can help you produce your results in record time.
 
As a special bonus, we will be showing a sneak peak of some of the new features coming down the road.
 


Fluidigm Corporation
7000 Shoreline Ct. #100
South San Francisco, CA 94080
Phone: 650-452-0638
Email: connie.cruz@fluidigm.com
Web: www.fluidigm.com

Room: South Hall 2B
Presenter: TBD

Thermo Fisher Scientific

102 Fountain Crescent
Inchinnan Business Park
Paisley, United Kingdom, PA4 9RE
Email:  jennifer.buick@thermofisher.com
Web:  www.thermofisher.com

Room: South Hall 2B
Presenter:  Jordi Petriz, PhD
Group Leader, Functional Cytomics Group
Josep Carreras Leukaemia Research Institute, Barcelona, Spain 

Title: Stream Oddities: Take Your Fluorescent Probes and Put the Acoustics On​

Acoustic Focusing Cytometers are analyzers that precisely align cells using ultrasonic waves prior to analysis with one or more lasers. The additional use of flat top profiles for the laser beam intensity allows for a better alignment even when super fast acquisition is used. Current implementations of acoustic focusing cytometers are also called as acoustic assisted hydrodynamic focusing cytometers, injecting acoustically pre-focused cells in the sample core stream into a sheath manifold, where they are further focused by sheath fluid. Acoustic focusing of cells in acoustically driven capillaries used for cytometry does not damage cells, making this technology available for rapid analysis of cells as well as to assist us in gaining understanding in the complex biology of the cells, origins, functions and activation states in combination with immunophenotypic markers.
I will discuss how the acoustic focusing technology can be applied to better understand the acoustic orientation effects with data collected over conventional fixed integrated angles of low angle forward and orthogonal side scatter. While many cells and microorganisms have similar scatter profiles contrast under hydrodynamic focusing, the acoustic focusing cytometers provide significantly different scatter signals for nearly identically shaped cells. Moreover, it will be discussed how the unique capabilities of acoustic focusing cytometry in association with 561 nm laser excitation can improve human hematopoietic counting protocols as well as to facilitate future clinical applications with complex multicolor panels, such as those including more than one functional cellular aspect in combination with cell immunophenotyping. I will also discuss how sample manipulation and red blood cell lysis can damage and provoke the loss of specific cells, as well as the benefits of using violet side scatter and no-lyse no-wash protocols for the identification of leukocytes, for rare cell detection in blood samples, as well as for sensitive detection of myeloid derived suppressor cells or small particles in fluids.
 

Wednesday, May 2

​1230-1330


Cytek Biosciences Inc. 
46107 Landing Parkway
Fremont, CA 94538
Phone: 510-657-0102
Email: greinin@cytekbio.com
Web: https://cytekbio.com/

Room: North Hall
Presenter: Dr. Maria Jaimes

Title: Expanding Application Capabilities Using Full Spectrum Cytometry
Flow cytometry has become a well-established tool in the fields of immunology and cell biology, but it presents some challenges. For example, it is challenging using flow for higher complexity panels. Additional lasers, custom filters, specialized optical configurations, and highly complex and expensive cytometers are required. Moreover, a high level of expertise is needed for panel design, and traditional methods for analyzing data from these panels are insufficient. It is also challenging to identify one cytometer that contains the application flexibility required by flow core labs. For example, special options or an application-specific cytometer are required for applications such as small particle detection. Additionally, flow applications such as microRNA prove challenging using a conventional cytometer due to the autofluorescent nature of cells. In this tutorial, we will present how high sensitivity full spectrum technology enables the Aurora cytometer to overcome these challenges and expand the breadth of applications that can be run on the same cytometer. Furthermore, we will demonstrate how the high-quality data produced can be used for principle component analysis of higher complexity panels. 


 
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