34th Congress of the International Society for Advancement of Cytometry
   

SATURDAY, 22 JUNE, 2019


SCIENTIFIC TUTORIALS 1-6
8:30 – 10:00

Spectral Unmixing and Compensation in Flow and Image Cytometry
David Novo, De Novo Software, USA 
Bartek Rajwa, Purdue University, USA


Genomic Cytometry: Using Multi-Omic Approaches to Increase Dimensionality in Cytometry
Rob Salomon, Institutute for Biomedical Materials and Devices (IBMD), University of Technology Sydney, Australia
Thomas Ashhurst, Sydney Cytometry Facility, The University of Sydney and Centenary Institute, Australia


Image Cytometry Fundamentals​
Calum MacAulay, BC Cancer Research Centre and University of British Columbia, Canada
Martial Guillaud, BC Cancer Research Centre and University of British Columbia, Canada


Data-Mining Techniques for Single-Cell Data
Yvan Saeys, VIB-Ghent University, Belgium

Funding an SRL
Franziska Grieder, National Institutes of Health, USA 
Kylie Price, Malaghan Institute of Medical Research, New Zealand


Moving from IP to Commercialization
Bill Hyun, University of California, San Franciso, USA 
Betsy Ohlsson-Wilhelm, SciGro, Inc./North Central Office, USA


COFFEE BREAK
10:00 - 10:30

SCIENTIFIC TUTORIALS 7-12
10:30 - 12:00

Multiplexing in Tissue Image Cytometry
Er Liu, Lyra Biomedical, Inc., USA

Best t-SNE on the Block: How to Achieve a Meaningful Low-Dimensional Embedding and Interpret it Correctly
Anna Belkina, Boston University, USA
Josef Spidlen, FlowJo, USA

Current Standards in Flow Cytometry Cell Sorter Biosafety
Geoffrey Lyon, Yale University, USA
Steve Perfetto, National Institutes of Health, USA
Evan Jellison, University of Connecticut Health Center


Deep Learning for Image Analysis -- WITHDRAWN
Devin Sullivan, KTH Royal Institute of Technology, Sweden

Bring It On! Challenging Samples Within an SRL Core, From A to Z: Acellular Organelles to Zooplankton
Nicole Poulton, Facility for Aquatic Cytometry, Bigelow Laboratory for Ocean Sciences, USA 
Rachael Sheridan, Van Andel Institute, USA

Panel Design – A Practical Guide for Successful Flow Cytometry Panels from 10-30 Parameters
Florian Mair, Fred Hutchinson Cancer Research Center, USA
Kelly Lundsten, BioLegend, USA

LUNCH
12:00 - 13:00

INSTRUMENTS4SCIENCE
12:00-13:00


SCIENTIFIC TUTORIALS 13-18
13:00 - 14:30
An Introduction to Imaging Flow Cytometry and its Data Analysis
Dominic Jenner, Defense Science Technology Laboratory, UK
Orla Maguire Roswell Park Comprehensive Cancer Center, USA


Photodetection in Flow Cytometry: Key Considerations for the Latest Detectors Including High QE PMTs, APDs, SiPMs and Cameras
Slawomir Piatek, New Jersey Institute of Technology, USA
Earl Hergert, Hamamatsu Corporation, USA
James Butler, Hamamatsu Corporation, USA

Advanced Data Analysis in a Shared Resource Laboratory
Sofie Van Gassen, VIB-UGent Center for Inflammation Research, Ghent University, Belgium
Dagna Sheerar, SCYM(ASCP), University of Wisconsin Comprehensive Cancer Center Flow Cytometry Laboratory, USA

Cell Image Classification: An Overview of Methods with Software Examples
Gustavo Rohde, University of Virginia, USA
Mohammed Shifat E. Rabbi, University of Virginia, USA

Writing, Publishing and Reviewing: Advice, Tips and News from Cytometry Part A – The Journal of Quantitative Cell Science
Attila Tarnok, University of Leipzig, Germany
Julia Kostova, John Wiley & Sons, USA

Flow Cytometry of Extracellular Vesicles: Guidelines for Reporting Methods and Results​
John Nolan, Scintillon Institute, USA
Joshua Welsh, National Institutes of Health, USA
Joanne Lannigan, University of Virginia, USA

CYTO INNOVATION
14:30 - 17:30
The advancement of cytometry depends on the translation of innovative research into useful, accessible tools that can catalyze new biological understanding. CYTO Innovation is the forum within ISAC that explores the challenges and opportunities for translation of new cell analysis technologies into commercially viable products and services. CYTO Innovation provides:

  • An opportunity to learn about the translation of ideas and discoveries into products and knowledge.
  • The skills required for progressing from a research finding to useful products with impact.
  • A unique platform for showcasing innovation in relevant industry sectors.
  • Engagement with a knowledgeable network of cytometry experts, entrepreneurs, executives and business development professionals.
CYTO Innovation is part of ISAC’s wider mission of advancing cytometry and aims to serve members and Congress attendees seeking to translate their research into high-impact products and services.  It is open to Full Congress Registrants; no additional fees apply. 

AGENDA:
14:30 – 14:40          Opening Remarks
Elena Holden, Chair, CYTO Innovation Committee
 
14:40 – 15:30          Keynote Presentation: “Starting a Biotech: Going       
                                   Over to the Dark Side or Seeing the Light”
                                   Allen Eaves, Founder, President and CEO of 
                                   STEMCELL Technologies Inc.


15:30 – 15:40           Coffee Break

15:40 – 16:30           CYTO Innovation Technology Showcase
 
1540-1545   Introduction, rules judges Elena Holden
1545-1550 Michael Stadinsky NovaFluors and InfiniFluors: Fluorescent Labels Engineered to Enhance Spectral Clarity and Multiplexed Detection Philtonex, Inc.
1550-1555 Russell Cole Microenvironment On Demand (MOD) - A Breakthrough Device For Immunotherapy Discovery Scribe Biosciences
1555-1600 Steven Graves A Revolution in High-Throughput Cell Analysis BennuBio Inc.
1600-1605 YongKeun Park Holotomography and Artificial Intelligence: From Label-Free Live Cells Imaging to Medical Applications Exploiting Cell Phenotypes Tomocube
1605-1610 Beverly Packard Quantitation of mRNA Levels in Live Cells OncoImmunin, Inc.
1610-1615 Yoke Khin Yap High-Brightness Dyes with Tunable Brightness StabiLux Biosciences, Inc
1615-1620 Nao Nitta AI Cell Sorter & Cell Analyzer K.K. CYBO
1620-1625 Kathrin Brenker Opto Biolabs -- Combining Optogenetics with Flow Cytometry Opto Biolabs
1625-1630   Technology showcase acknowledgements and closing  

16:30 – 17:10           Panel Discussion
“Experience is something that you get just after you need it”.
 
An expert panel will expound on the challenges faced by those engaged in innovation starting from academia and business. The panel will draw upon their collective experiences in founding and leading companies that impact on the cell-based sciences and unmet needs.

Moderator: Paul Smith, Biostatus.
Panelists: Allen Eaves, STEMCELL Technologies; Michael Stadnisky, FlowJo;   Bill Hyun, UCSF and Biotech Entrepreneur; James Taylor, Precision NanoSystems; Ryan Brinkman, BC Cancer Agency.

17:10 – 17:30           CYTO Innovation Awards and Networking

SRL NETWORKING EVENT
17:30 - 19:00

AGENDA
Welcome & Introduction
  • Preview of the events by the organizers - ISAC SRL Emerging Leaders Aja Rieger, Diana Bonilla, Claudia Bispo, Vera Tang, Gelo Dela Cruz
Presentation: SRL Communication Publications in Cytometry Part A Presentation: CYTO U and ISAC Educational Initiatives
  •    Joanne Lannigan: Director, Flow Cytometry Shared Resource, University of Virginia Discussion & Table Networking
Panel Discussion: Your most challenging issues and how you are addressing it
  • Lead by ISAC President-Elect Jonni Moore with other experienced SRL Leaders
Networking/Social
  •         Make lasting connections with fellow Shared Resource Lab staff, managers, and directors through networking activities and open networking/social time.
Advance registration is required and seating is limited. Ticketed attendees will be admitted on a first come, first served basis until room capacity is filled.

SUNDAY, 23 JUNE, 2019

FIRST TIME ATTENDEES ORIENTATION
7:30 - 8:30

STATE OF THE ART LECTURES: The Leading Edge of Cytometry and Informatics
8:30 - 10:00

Translating a Trillion Points of Data into Therapies, Diagnostics, and New Insights into Immunology​
Atul Butte, UCSF, San Francisco, California, USA

Cell Image Classification: Methods and Applications
Gustavo Rohde, University of Virginia, USA

High Parameter Flow Cytometry and a New Computational Platform Reveal Unique Cell Phenotypes that Predict Melanoma Outcomes  
Pratip Chattopadhyay, Isaac and Laura Perlmutter Cancer Center
NYU-Langone Medical Center, USA


COFFEE BREAK
10:00 - 10:30

CONCURRENT PARALLEL SESSIONS 1-4
10:30 - 12:00

Parallel Session 1: Application of High Parameter Cytometry to Disease I

Multi-Dimensional Functional Profiling Of Human Rhinovirus-Specific T-Cells By Means Of Spectral Flow Cytometry, Alberta Paul, et. al. 

Multi-omic single-cell analysis of the human antigen-presenting cell  compartment reveals tissue- and tumor-specific specialization, Florian Mair, et. al.

A computational pipeline for the diagnosis of CVID patients, Annelies Emmaneel, et. al.,
 
Differential dynamics of the maternal immune system in healthy pregnancy and preeclampsia, Xiaoyuan Han, et. al.
 
Parallel Session 2: New Algorithms I
 
Multiview Machine Learning Increases Predictive Power in Clinical Flow and Mass Cytometry Datasets, Natalie Stanley, et. al.
 
Deep Cytometry Embedding (DiCE): a deep-learning approach for display and interrogation of multi-relational flow cytometry data, J. Paul Robinson, et. al.
 
Characterising immune cell development in West Nile Virus infection using clustering and tracking algorithm, Givanna Putri, et. al.

A  Computational  Approach  for  Automated   Biomarker   Discovery:   Identification   of   cell subpopulations involved in acute response to Chikungunya virus with CITRUS in Cytobank, Geoff Kraker, et. al.
 
Parallel Session 3: New Technologies I
  

Molecular Cytometry: Optimization, Characterization, and Application to Tumor Immunology, Pratip Chattopadhyay, et. al.
 
Parallel flow cytometry simplified, Steven Graves, et. al. 
 
A High-Throughput Flow Cytometry Platform for Precision Drug Development Identifies Avenues for Improving Immunotherapy Targeting, Diane Heiser, et. al.
 
Personal 1.5 kg Microvolume Photon Counting Cytometer for Cell and Biomarker Analysis, Eugene Chan, et. al.
 
Parallel Session 4: Unique Instrumentation and Approaches
    

Rectangular flattop beam shaper for multi-wavelength lasers using a diffractive optical element, Jingjing Zhao, et. al. 
 
Repeated flow cytometry measurements along a single microfluidic channel in an optofluidic chip, Gregory Cooksey, et. al. 
 
Spheroid Painting with DRAQ9: Simple Identification and Measurement in 3D Cell, Rachel  Errington, et. al.
 
Combined high-throughput single-cell DNA genotyping and protein quantification, Ben Demaree, et. al.

LUNCH
12:00 - 12:30

COMMERCIAL TUTORIALS
12:30 - 13:30


Beckman Coulter Life Science
5350 Lakeview Parkway
Indianapolis, IN 46268
Phone: 714-342-9074
Web: www.beckmancoulter.com

Presenter: Karla Williams, Ph.D., Assistant Professor at the University of British Columbia Faculty of Pharmaceutical Sciences

Extracellular Vesicles for ‘Liquid Biopsy’ Development in Cancer
 

The use of extracellular vesicles (EVs) as biomarkers is a rapidly expanding field with great promise and clinical potential to report on tumors and their heterogeneity. The advantages of using EVs over cells and circulating tumor DNA is their abundance and stability.  EVs are rapidly shed from tumor cells either directly from the plasma membrane (micovesicles) or through exocytosis (exosomes) and are readily detectable in the blood (~1–3 × 1012 exosomes per ml of plasma). The cargo contained within the EV is representative of the cell of origin containing lipids, proteins, glycans and nucleic acids. Direct enumeration of tumor-derived EVs and/or profiling of their molecular cargo in patient body fluids have been shown to provide valuable information about the biology of the tumor.  We are using nanoscale flow cytometry to identify tumor-derived EVs and profile their protein and glycan composition. This has led to the discovery of a novel glycan present on the surface of prostate derived EVs. From this work we are developing blood-based ‘liquid biopsy’ to risk-stratifying patients, distinguish indolent from aggressive disease.

Sony Biotechnology Inc.
1730 North First St. 
San Jose, CA 95112
Phone: 800-275-5963
Fax: 408-352-4130
Email: jordan.mackinnon@sony.com
Web: http://www.sonybiotechnology.com
 
Presenter: Deena Soni, PhD
 
Single Cell Isolation for RNA sequencing analysis- Challenges and Applications

Cell sorting is increasingly used to isolate single cells for a variety of downstream applications. In recent years, single cell RNA sequencing analysis has emerged as a very important tool used across life science disciplines spanning from cancer biology to microbiology. It has enabled researchers to profile rare populations, uncover relationships between genes, and trace distinct cell lineages in development. Identification and efficient isolation of the cells of interest is a critical step in obtaining accurate information of the transcriptome of individual cells. 
In this tutorial, we will discuss how flow cytometry based cell sorting has become an important approach for isolating highly purified single cells with variable marker abundance. We will discuss the approaches used for isolating a variety of cell types using the Sony cell sorter. This system is an easy to use solution for users of all expertise levels and can be used to characterize and isolate cells for screening unique transcripts in hundreds of cells.
 
Thermo Fisher Scientific
5791 Van Allen Way
Carlsbad, CA 92008
Phone: 716-774-6735
Email: info@thermofisher.com
Web: http://www.thermoscientific.com/nanodrop
 
Presenter: Colin Thalhofer, Ph.D., Research Scientist, AgonOx, Inc.

Tumor Specific CD8 T cell Identification, Expansion, and Clinical Research Implications

Through flow cytometry analysis, cell sorting and in vitro techniques, AgonOx has developed a workflow for detecting, isolating and expanding endogenous tumor antigen-specific CD8 T cells from individuals own tumor tissue (Duhen, et al, Nature Communications, volume 9, Article number: 2724 (2018)).  The highly enriched tumor-reactive T cells are grown in vitro with a method optimized to achieve large cellular expansion and to maintain a broad repertoire of tumor-reactive T cell clones.  The expanded T cell product typically retains much of its original T cell clonal diversity and preserves or reinvigorates its tumor-lytic potential.  This platform yields a uniquely personalized subject-derived product for individuals from a wide variety of tumor types.  This tumor-specific T cell product is specific/personalized for each subject and has clinical research applications in the Immune Oncology field.
 
Fluidigm Corporation
7000 Shoreline Court Suite #100
South San Francisco, CA 94080
Phone: 650-266-6000
Email: Matt.Kirtley@fluidigm.com
Web: www.fluidigm.com
 
Presenters: Michelle Poulin, PhD, Manager, Field Applications, Fluidigm; Thomas Ashhurst, BSc, High-Dimensional Cytometry Specialist, Sydney Cytometry Facility, University of Sydney
 
Deep profiling of immune cell phenotypes and function from circulation to tissue with  CyTOF® technology
 
Highly multiplexed mass cytometry and Imaging Mass Cytometry™ (IMC™) are transforming our understanding of the immunome. After a short introduction, Michelle Poulin, PhD, will present The new standard for deep immune profiling with CyTOF® technology: 30 markers, 1 tube, 5-minute data analysis. She will describe the Maxpar® Direct™ Immune Profiling System, an exciting new product providing a sample-to-answer solution that enables enumeration of 37 immune cell populations from human PBMC and whole blood. Data on repeatability, software precision and accuracy, and site-to-site reproducibility will be shown. Then, Thomas Ashhurst, BSc, will present Investigating immune infiltration during viral encephalitis using Imaging Mass Cytometry, highlighting his research on how viral infection of the central nervous system stimulates a rapid influx of bone marrow-derived monocytes/macrophages into the parenchyma. He will describe use of IMC to perform high-dimensional tissue profiling to gain a more comprehensive analysis of the key factors involved in this mobilization of the immune system.

Luminex Corporation
12212 Technology Boulevard
Austin, TX 78727
Phone: 512-219-8020
Fax: 512-336-3530
Email: info@luminexcorp.com
Web: http://www.luminexcorp.com

Presenter: Haley Pugsley, PhD, Senior Scientist Luminex

High Sensitivity Flow Cytometry Enables Extracellular Vesicle Immunophenotyping

The Amnis® CellStream™ benchtop flow cytometry system from Luminex is a highly-customizable and compact flow cytometer that is the first to use a camera for detection. Its unique optics system and design provides researchers with unparalleled sensitivity and flexibility when analyzing cells and submicron particles. 

Only recently has the importance of extracellular vesicles as key mediators of intercellular communication been appreciated. Quantifying and characterizing extracellular vesicles in a reproducible and reliable manner has been difficult due to their small size (30 – 100 nm in diameter). 

Join us in this workshop to learn more about how the unique Amnis® time delay integration (TDI) and camera technology inside the CellStream™ system enables immunophenotyping of extracellular vesicles.

Summary:  The Amnis® CellStream® benchtop flow cytometry system from Luminex provides researchers with unparalleled sensitivity and flexibility when analyzing cells and submicron particles.  The research presented in this talk demonstrates the use of the CellStream to immunophenotype extracellular vesicles. 

Menarini Silicon Biosystems
3401 Masons Mill Road, Suite 100
Huntingdon Valley, PA 19006
Phone: 215-346-8200
Email: vvarghese@siliconbiosystems.com
Web: http://www.siliconbiosystems.com/
 
Presenter: Arielle Ginsberg, MSc, Scientific Affairs Liaison 
 
DEPArray™ Image assisted Digital Cell Sorter enables isolation of 100% pure single cells in samples with rare targets and very low cell count. 

The DEPArray™ platform uses digital sorting technology to enable the isolation of single or groups of cells with unmatched precision and purity. 100 to 30,000 cells are introduced into the flow cell of a microfluidic/microelectronic chip and trapped in dynamically controlled dielectrophoretic (DEP) cages formed by hundreds of thousands of microelectrodes that are distributed on the floor of the flowcell. Once immobilized in cages, cells are identified using a combination of immunofluorescent staining and optical imaging. Selected cells are then precisely isolated by electronically moving individual DEP cages containing the captured cells of interest, leading them to a collection tube. The digital precision of this technology allows the isolation of even one single cell from up to 30,000 loaded on the chip with no compromise on purity, enabling the most challenging rare cell sorting applications. Among the most challenging demonstrated applications, the isolation of circulating tumor cells (CTC) from enriched blood, isolation of Reed Sternberg cells (RSc) from classic Hodgkins Lymphoma FFPE archived samples, and isolation of single blood cells from mixed blood stains for forensic identification of each contributor.

Cell Microsystems
2 Davis Dr 
PO Box 13169 
Research Triangle Park, North Carolina 27709
Email: ntrotta@cellmicrosystems.com 
Web: http://www.cellmicrosystems.com

Presenter: Nick Trotta, PhD, Director of Genomics Programs

Imaging-based cloning using the CellRaft AIR System for verifiable clonality, high viability and hands-free efficiency ​
 
Cell Microsystems provides rapid and efficient cell sorting with the automated AIR™ System.  Cells are cultured and imaged on the CytoSort Array, a microwell culture plate featuring thousands of releasable CellRafts for gentle and trackable cell isolation.  Single cell suspensions, seeded on the CytoSort Array, can be imaged, analyzed and isolated on the computer-controlled AIR™ System.  Both single cell analysis and clonal propagation are supported by the CellRaft workflow.  During the tutorial, we will provide case studies and methods for the isolation of single cells as well as clonal colonies. Viability of sorted cells has been shown to be at least double compared to other sorting technologies.  Cells can also be imaged and sorted based on detailed subcellular features or dynamic phenotypes which require observation over hours or days.  Application-specific data will also be presented, such as CRISPR gene editing and iPSC workflows. New products, such as the HexaQuad Array which allows 24 distinct cell populations to be cultured, imaged and sorted, as well as new software, permitting sorting of single cells and colonies using only brightfield imaging, will also be introduced.  

PLENARY SESSION 1: 3D Cytometry
13:30 - 15:00

"Google Maps" for Tissue Biology – How to Find Topographic Biomarkers?
Denis Schapiro, Harvard Medical School and Broad Institute

Quantitative Phase Imaging and Artificial Intelligence: Label-Free 3D Imaging and Analysis of Individual Live Cells
YongKeun (Paul) Park, KAIST, South Korea

Intelligent Image-Activated Cell Sorting
Keisuke Goda, University of Tokyo, Japan

COFFEE BREAK
15:00 - 15:30

CONCURRENT WORKSHOPS 1-5
15:30 - 16:30

Creating a Repository of Best-Practice Guidelines for Successful Cell Sorting Outcomes
Facilitators: Gelo dela Cruz, Peter Lopez, Anna Fossum

Strategies for Successful Conflict Resolution: You Can't Hide in Your Office and Hope the Problem Goes Away
Facilitators: Jessica Back, Ann Marie Deslauriers-Cox

The Status of Flow Cytometry in Africa
Facilitators: Lydia Tesfa, Alfonso Blanco, Bill Telford, Iyadh Douagi 
Speakers: Lydia Tesfa, Alfonso Blanco, J. Paul Robinson, Bill Telford, Iyadh Douagi​

Applying Scatter & Fluorescence Standardization to Flow Cytometric Data of Small Particles
Facilitators: Joshua Welsh, Vera Tang

How to Ensure Robustness and Reproducibility when Using Mass Cytometry for Clinical Studies?
Facilitators: Jose Estevam, Diana L. Bonilla Escobar, Holden Maecker, Adeeb Rahman, Jonathan Irish, James Lederer, Brice Gaudillière, Nima Aghaeepour, Elena Hsieh Professor, Greg Behbehani, Mike Leipold, Radhika Rayanki, Peter Krutzik, Chris Groves

FUTURE SCAN: A Panel Discussion About the Future of Cytometry
16:30 - 17:30

EXHIBITS OPEN
18:00 - 20:00

OPENING RECEPTION
17:30 - 18:30


MONDAY, 24 JUNE, 2019

POSTER VIEWING HOURS
8:00 - 18:00

FRONTIERS SESSION 1: New Frontiers in Single Cell Biology: Epigenetics and Metabolomics
8:30 - 10:00

Single-Cell Epigenomics in Cancer Immunotherapy

Ansuman Satpathy, Stanford University, USA

T Cell Metabolism and Fate
Jeff Rathmell, Vanderbilt University Medical Center, USA

EXHIBITS OPEN
10:00 - 18:00

COFFEE BREAK
10:00 - 10:30

CONCURRENT PARALLEL SESSIONS 5-9
10:30 - 12:00

Parallel Session 5: Application of Multimodal Analysis to Disease Biology    

Single Cell Systems Neuroimmunology Reveals Immunosuppressive Correlates with Ventricular Stem Cell Niche Contact in Human Glioblastoma, Todd Bartkowiak, et. al.

High risk glioblastoma cells revealed by machine learning and single cell signaling profiles, Jonathan Irish, et. al.
 
Using single cell systems immunology approaches to decipher phagocyte heterogeneity, signaling and transcription profiles in colorectal cancer, Maunish Barvalia, et. al. 
 
Mutimodal single-cell analysis identifies a novel pro-resolving macrophage subpopulation following acetaminophen-induced liver injury, Dyana Markose, et. al.

Parallel Session 6: New Sorting News I

Computational Sorting with HyperFinder, Nikolay Samusik, et. al.
 
Vortex-Actuated Cell Sorting (VACS): single stream and parallelisation, Salman Samson Rogers, et. al.
 
High throughput fluorescence lifetime and dual color fluorescence activated droplet microfluidic sorter, Sheng-Ting Hung, et. al. 
 
The S6 Cell Sorter – A Novel 30-Parameter High Speed Cell Sorter, Stephen Perfetto, et. al.
 
Parallel Session 7: 3D and Spatial Profiling of Cells and Tissue  

Immune-tumor spatial architecture in breast cancer subtypes, Jennifer Eng, et. al.
 
Ultrafast label-free imaging cytometry enables massive and in-depth single-cell biophysical phenotyping, Dickson M. D. Siu, et. al.
 
Intravital three-photon microscopy, Raluca Niesner, et. al. 
 
Label-free High-Content SRS Image Cytometry for Rare Population Analysis at Sub- cellular Resolution, Kai-Chih Huang, et. al.
 
Parallel Session 8: New Technologies II   

Application of Enzyme-assisted Signal Amplification in Upconversion Resonance Energy Transfer for DNA Detection and Quantification, Yinghui Chen, et. al.

Combining optogenetics with flow cytometry, Kathrin Brenker, et. al.

Point-of-care 24/7 load & go flow cytometry in determining innate immune responses in trauma patients: the start of a new era, Roy Spijkerman, et. al. 
 
Applying novel technologies for development of a sensitive liquid biopsy for transcriptomic single- cell profiling of circulating Multiple Myeloma cells, Ling Wang, et. al.
 
Parallel Session 9: Small Particle Analysis    

Development of a flow cytometer for nanoparticle detection, Martin Hussels, et. al.  
 
Bioprocessing of Nanoparticles and Extracellular Vesicles (EVs) in a Dynamic Cell System, Timothea Konstantinou, et. al.
 
Ultrafast microfluidic cell compressions for intracellular delivery of large macromolecules and particles, Alla Zamarayeva, et. al.
 
Engineered retroviruses as fluorescence reference particles for nanoscale flow cytometry, Vera Tang, et. al.

LUNCH
12:00 - 12:30

COMMERCIAL TUTORIALS
12:30 - 13:30


Sysmex America
577 Aptakisic Road
Lincolnshire, IL 60069
Phone: 224-543-9676
Email: info@sysmex.com
Web: www.sysmex-partec.com/

Advances in Multi-parameter Flow Cytometry Data Analysis and New Detection Systems for Yeast and Bacteria
Taking full advantage of multi-core processors, the latest version of VenturiOne® software will be presented, including examples of new features to further improve and quicken the analysis of complex, multi-parameter data sets produced by the Sysmex XF-1600™ or virtually any other flow cytometer.
The second section of the tutorial will concentrate on new assays for microbiology. Flow cytometric detection of living Alicyclobacillus spp. as well as Alicyclobacillus acidoterrestris cells in fruit juices, fruit concentrates and similar samples, in addition to living Brettanomyces bruxellensis cells in wine and pre-mature wine samples, is now possible. Specifically programmed gene probes bind to target structures within the corresponding living bacteria or yeast to deliver maximum specificity and speed for their detection.
For research use only. Not for in vitro-diagnostic or therapeutic use
 
BD Biosciences
2350 Qume Dr. 
San Jose, CA 95131
Phone: 877-232-8995
Fax: 408-954-2009
Email: answers@bd.com
Web: www.bdbiosciences.com
 
Presenters: Robert Balderas, VP, Biological Sciences and Maggie Nakamoto, Senior Scientist, BD Biosciences
 
Harnessing Multiomic Data in Single Cells for Discovery
 
High-throughput single cell RNA sequencing (RNA-seq) has recently emerged as a powerful tool for profiling complex cell populations. While traditional RNA-seq captures information about transcript expression, recent technological advances using oligo-conjugated antibodies enable simultaneous detection of protein alongside RNA in high-throughput sequencing. This technology enables high parameter protein analysis, and the addition of protein information to RNA-seq data can provide better resolution of markers with low transcript abundance. This also enables more precise cell type identification and more intricate separation of cell types in a complex system. Ultimately, combining information from whole-transcriptome RNA-seq (WTA), targeted gene panels and protein profiling (BD® AbSeq) can generate novel insights into single cell biology. Here, we discuss the interplay between single cell sequencing multiomic data and other technologies, as well as how best to harness the information gained from high parameter single cell sequencing data.
 
Thermo Fisher Scientific
5791 Van Allen Way
Carlsbad, CA 92008
Phone: 716-774-6735
Email: info@thermofisher.com
Web: http://www.thermoscientific.com/nanodrop
 
Presenter:  Jolene A. Bradford, MLS, SCYM(ASCP)TM, Associate Director, Protein and Cell Analysis, Thermo Fisher Scientific, Eugene, Oregon USA

Novel Fluorescent Probes and Labeling Strategies for Flow and Imaging Applications

Invitrogen™ has developed a number of novel reagents and assays designed to accelerate the ability to characterize cell health and function. This seminar will present recently developed assays for the detection of hypoxia, senescence, and apoptosis with use in both flow cytometry and imaging based applications.  Over the past decade, biologics have been increasingly pursued as therapeutic agents. In the case of antibody–drug conjugates (ADCs), internalization of the antibody is a powerful mechanism of action. Internalization moves the ADC from its binding site at the plasma membrane of the target cell to the lytic environment of the lysosome, resulting in activation of the attached toxin. With the A to Z of antibody labeling strategies presented as background, antibody labeling tools to measure internalization efficiency will be highlighted.  For research use only. Not for use in diagnostic procedures.

ACEA Biosciences, Inc.
6779 Mesa Ridge Road Ste. 100
San Diego, CA 92121
Phone: 858-724-0928
Email: info@aceabio.com
Web: www.aceabio.com
 
Presenter: Dr. Mikkel Steen Petersen and Dr. Garret Guenther
 
Integrated Flow Cytometry Solutions More Accessible Than Ever - Recent Advances in NovoCyte® Flow Cytometry Platform
 
Abstract:  In this workshop, our two speakers will introduce the latest advances in flow cytometry applications and solutions enabled by NovoCyte® platform from Agilent.
 
The new high-performance NovoCyte platform now provides the seamless 1~4 laser and 3~25 color upgradability and flexibility with optional Flow automation capability. As a part of Agilent’s cell analysis technology platforms, NovoCyte benchtop flow cytometers for all laboratory settings are made accessible more than ever to enable breakthrough discoveries by meeting the needs of most diverse and demanding flow cytometry applications. Meaningful access to flow cytometry results via laboratory information management systems (LIMS) is a key to diagnostic and therapeutic decisions. LIMS solutions in the market are too expensive, difficult to implement or maintain to accommodate flow cytometric data and workflow. Our guest speaker will illustrate how to "build-your-own" flow cytometry laboratory information management system with NovoCyte Flow instruments, NovoExpress® data collection and analysis software, and a generic low-cost database software (FileMaker) with an outline of the steps requiring little to none programming experience.
 
Attendees will learn how to apply the integrated flow cytometry solutions into their own cellular analysis workflows.
 
De Novo Software
400 North Brand Blvd, Suite 850
Glendale, CA 91203
Phone: 213-814-1240
Fax: 213-814-1240
Email: chris.concannon@denovosoftware.com
Web: www.denovosoftware.com
 
Presenter: Dr. David Novo
 
What's New In FCS Express 7

De Novo Software is proud to introduce FCS Express 7. Version 7 builds upon 20+ years of experience and  brings new visualizations for experiments, easier ways to distill highly multi-parametric data sets, more speed and many other improvements that facilitate getting results more quickly. Version 7 will be exclusively available via a new subscription licensing model allowing users to easily have access to the latest features.
 
FCS Express Version 7 continues to move beyond other software packages, which  focus on generating plots, gates and stats that are rarely your final results. FCS Express 7 has a wide variety of tools to bring your final results to you directly in your flow analysis software including spreadsheets, live updating statistical tests like T-test and ANOVA and regressions that link directly to your gates. Skip tedious exports to Excel and leave copy and paste behind. Come get your first look at Version 7 and see how FCS Express can help you produce your results in record time.
 
Bio-Rad Laboratories
2000 Alfred Nobel Drive
Hercules, CA  94547
Phone: 510-741-4486
Email: sonya_sano@bio-rad.com
Web: http://www.bio-rad.com

Presenters:
Karen Helm, Flow Cytometry Shared Resource, University of Colorado Cancer Center
David Adams, Managing Director of Flow Cytometry Core at University of Michigan

 
Using the ZE5 Cell Analyzer to Improve the Efficiency of Shared Resource Laboratories

Join Bio-Rad and some of the core labs running the ZE5 Cell Analyzer as they share how the ZE5 Cell Analyzer meets the unique needs of scientists for high-throughput and multi-parameter analyses.

PLENARY SESSION 2: Single Cells in Translation
13:30 - 15:00 
Tfh Response to Flu Vaccine​
Ramin Herati, University of Pennsylvania, USA

Flow Cytometric Applications for Bacterial Pathogenesis​
Alison Criss, University of Virginia, USA

Automated Image Classification Using Deep Learning
Aristotelis Tsirigos, New York University School of Medicine, USA

COFFEE BREAK
15:00 - 15:30

CONCURRENT WORKSHOPS 6-11
15:30 - 16:30

User Training in Cell Sorting in Core Facilities – Eliminating the Staff to Instrument Bottleneck
Facilitators: Christina Ewald, Derek Davies, Claudia Dumrese

Finding Solutions to Difficult Developmental Biology and Stem Cell Flow Cytometry Issues
Facilitator: Gelo dela Cruz

SRL Careers: What Can ISAC Do to Help Us?
Facilitators: Yasmin Hacque, Rachael Walker, Aja Rieger, Julfa Begum, Kylie Price

When the Shoe Doesn't Fit: Defining and Overcoming Barriers to Achieving Best Practices for Solo-Staff SRLs
Facilitators: Roxana del Rio-Guerra, Laura Lewis-Tuffin

Flow Cytometry Data for Clinical Trials, Manual or Automated Analysis?
Facilitators: Michael Nathan Hedrick, Yongliang Steve Sun, Cherie Green, Ryan Brinkman

CYTO Lab Hacks: A Platform for the Exchange of Innovations in Cytometry
Facilitators: Jakub Nedbal, Claudia Bispo, Christopher Hall, Bunny Cotleur

POSTER SESSION 1 PREVIEW: 5-Minute Flash Talks Describing the Highest Scoring Posters
16:30 - 16:45

POSTER SESSION 1
16:45 - 18:00

HAPPY HOUR
16:45 - 18:00

TUESDAY, 25 JUNE, 2019

POSTER VIEWING HOURS
8:00 - 18:00

ROBERT HOOKE LECTURE
9:00 - 10:00

Normal and Neoplastic Stem Cells​
Irv Weissman, Stanford University, USA

EXHIBITS OPEN
10:00 - 18:00

COFFEE BREAK
10:00 - 10:30

CONCURRENT PARALLEL SESSIONS 10-14
10:30 - 12:00

Parallel Session 10: Application of High Parameter Cytometry to Disease II  

High-dimensional single-cell profiling identifies an effector Treg molecular program governing human lung cancer disease progression, Enrico Lugli, et. al.

Exploring immune cell dynamics in inflammatory bowel disease and its therapeutic modulation by TNF-alpha blockade, Sabine Baumgart, et. al.

Using High Parameter Cytometry to Delineate and Characterise Microglial and Infiltrating Inflammatory Monocyte Response Kinetics in West Nile Virus Encephalitis, Alanna  Spiteri, et. al.
 
The influence of heritable and environmental factors on immune homeostasis: An approach to elucidate mechanisms of autoimmunity and infectious disease susceptibility, Thomas Liechti, et. al.

Parallel Session 11: New Imaging Technologies

Highly sensitive imaging flow cytometry enabled by optomechanical image acquisition with a high-speed camera, Hideharu Mikami, et. al.

Image-free "Imaging" Flow Cytometry, Toru Imai, et. al.
 
Label-free molecularly specific flow cytometry and beyond, Kotaro Hiramatsu, et. al. 
 
The role of mitochondria, fission, fusion and tunneling in U87 MR glioblastoma cell line proliferation using quantitative, label free, holographic and tomographic time lapse analysis, Ed  Luther, et. al.
 
Parallel Session 12: New Algorithms II
 

Machine Learning for Integration of Mass Cytometry with the Transcriptome, Microbiome, Proteome, and Metabolome for Prediction of Clinical Outcomes, Mohammad Sajjad Ghaemi, et. al.
 
Syzygy Forces: Using Immune Cell Networks to Reverse Immune Suppression in Spaceflight and Melanoma, Michael Stadnisky, et. al.
 
Grow your own gating tree: Using machine learning to create and forward propagate a gating structure for exploratory and diagnostic methods, Christoph Freier, et. al.
 
Cell-Type Specific Subspace Clustering Analysis of High-Dimensional Flow and Mass Cytometry Data for Data-Driven Identification of Difficult-to-Resolve Cell Populations, Yu Qian, et. al.
 
Parallel Session 13: Immune Landscapes


From bone marrow to peripheral blood: incidence of the G-CSF-mediated mobilization on cellular landscape, Lea Guyonnet, et. al.
 
Deep Profiling of the Neonatal Immune System using CyTOF, Laura Peterson, et. al. 
 
Not only gene mutation matters: Development and validation of flow cytometry panel to determine BRCA1/2 deficiency and sensitivity to personalized therapy by PARP inhibitors in cancer, Kasia Piwocka, et. al.
 
Bioprosthetic Total Artificial Heart implantation induces mobilization of non-KDR circulating  stem and progenitor cells in peripheral blood, Coralie Guerin, et. al.
 
Parallel Session 14: Standardization, Performance, Optimization

A Modified Injector and Sample Acquisition Protocol Improves the Data Quality and Reduces the Inter-Instrument Variability of the Helios Mass Cytometer, Brian Lee, et. al.

Flow Cytometer Characterization and Standardization in the HIV Vaccine Clinical Trials Network, Katharine Schwedhelm, et. al.
 
Resolving spectra and resolving populations: quantifying performance in spectral flow cytometry, Peter Mage, et. al.

Reproducibility Project in Cancer Biology: Microscopy image acquisition, processing and analysis perspective on replication study no. 20, Judith Lacoste, et. al.

LUNCH
12:00 - 12:30

COMMERCIAL TUTORIALS
12:30 - 13:30


Beckman Coulter Life Science
5350 Lakeview Parkway
Indianapolis, IN 46268
Phone: 714-342-9074
Web: www.beckmancoulter.com
 
Presenter: Denis Polančec

The Difference Between Stromal Vascular Fraction Cell Content  in Microfragmented Lipoaspirate Compared to the Lipoaspirate Counterpart

Aim:  The aim of the study was to optimise the protocol for the isolation of the stromal vascular fraction cells (SVFC) from lipoaspirate obtained by classic lipoaspiration or microfragmented lipoaspirate, as well as to compare the immunophenotypic profile of each SVFC by flow cytometry.
 
Materials and Methods:  Lipoaspirate samples were obtained from 10 patients with knee osteoarthritis for a head-to-head comparison between lipoaspirate and microfragmented lipoaspirate. After SVFC isolation by a gentle collagenase digestion, cells were counted and stained using a prototype of the DuraClone mesenchymal stem cells (MSC) tube (Beckman Coulter, USA) for the detection of the cell surface markers: CD31, CD34, CD45, CD73, CD90, CD105, CD146. Dead cells and the cell nuclei were stained with Live/Dead Yellow Fixable Stain (ThermoFisher, USA) and DRAQ7 dye (Beckman Coulter, USA), respectively. The Kaluza software (Beckman Coulter, USA) was used for the analysis of FCS data files.
 
Results:  In both type of samples, five major subpopulations were identified: CD45+ cells (leukocytes) and within the CD45- fraction endothelial mature cells (EM; CD31+CD34-), endothelial progenitor cells (EP; CD31+CD34+), pericytes (CD31-CD34±CD146+) and supra-adventitial adipose stromal cells (SA-ASC; CD31-CD34+CD146- ). The EP cells, pericytes and SA-ASC showed different expression pattern of the CD73 and CD90 mesenchimal stromal cell markers. The relative amount of EP cells and pericytes in microfragmented lipoaspirate were higher compared to lipoaspirate, while relative amount of the SA-ASC and CD45+ cells were lower.
 
Conclusions:  The Duraclone MSC prototype tube was successfully used for the immunophenotyping of the SVFC from lipoaspirate and microfragmented lipoaspirate tissue by flow cytometry. The fact that SVF from microfragmented lipoasirate samples is enriched in EP cells and pericytes, with a concomitant reduction of leukocytes, holds an explanatory potential for its anti-inflammatory and regenerative properties.

BD Biosciences
2350 Qume Drive
San Jose, CA 95131
Phone: 408-954-6026
Email: carrie.carruthers@bd.com
Web: www.bdbiosciences.com
 
Presenters: Robert Balderas, VP, Biological Sciences and Geoffrey Osborne, Director Special Order Research Products, BD Biosciences
 
Breaking Through the 28-Color Barrier

Fundamental immunology has benefited greatly from flow cytometry advances. In particular, contributions from receptor biology have led to a deeper understanding of cellular protein composition, phenotype and function. Simultaneously, discoveries and advances in genomics, proteomics and other –omics fields have exposed the need to recover specific, functional cell subsets in order to extract novel insights. However, as we gain a deeper understanding of biology, our instrumentation and data analysis tools are also becoming increasingly complex. We seek to develop workflows that harness these increasingly sophisticated hardware and software approaches in streamlined, simplified workflows.
We will discuss advances in BD’s high parameter platform solutions for analysis and sorting with the BD FACSymphony™ A5 and S6 systems. These advances include:
•  Complementarity of the high-dimensional analyzer and sorter platforms
•  A simplified real-time spectral compensation workflow
•  HyperFinder: A sorting workflow that implements computational gating and provides objective measurement of performance

Miltenyi Biotec GmbH
Friedrich-Ebert-Straße 68
Bergisch-Gladbach 51429
Germany
Phone: +49 2204 830630
Fax: +49 2204 85197
Email: macs@miltenyibiotec.de
Web: www.miltenyibiotec.com
 
Presenters: Dr. Stefan Eulitz and Dr. Kalpana Singh
 
The Easy Way to Ultrahigh-Content Imaging
 
Biological systems and cellular processes are inherently complex due to the interaction of thousands of proteins involved in proper functioning of the entire organism. Thus, an in-depth analysis of biological systems requires the examination of a plethora of parameters in order to decipher the underlying principles. Currently available techniques can provide only a very limited perspective on the complexity of biological systems. Here we introduce the MACSima™ Imaging Platform which impressively overcomes these limits as it allows the microscopic analysis of hundreds of proteins on a single sample, multiple samples a time, in a completely automated system. Antibodies play a key role for all cell analysis applications but so far only few technological innovations have been introduced to enhance antibody functionalities. Miltenyi Biotec offers a broad portfolio of standardized, lot-to-lot consistent, recombinantly engineered antibodies validated for flow cytometry, multiparameter high-content imaging, and light sheet microscopy. The recombinant antibodies are engineered to eliminate background signal, show flexible affinity, and are offered as direct conjugates to provide a perfect balance between signal strength and sensitivity.

Luminex Corporation
12212 Technology Boulevard
Austin, TX 78727
Phone: 512-219-8020
Fax: 512-336-3530
Email: info@luminexcorp.com
Web: http://www.luminexcorp.com
 
Presenters: Neil Bauersfeld, Senior Software Manager and Vidya Venkatachalam, PhD, Director, Software and Algorithms R&D
 
Introducing two software modules that bring new functionality in image acquisition and analysis software from Amnis, now a part of Luminex Corporation
 
The ImageStream® Mk II and FlowSight® imaging flow cytometers deliver objective, quantitative and statistically significant high-content data to inform decisions regarding cellular processes, drug discovery, drug development and basic research questions. To address the need to control and track data acquisition and analysis for regulatory compliance, we are introducing new versions of data acquisition and analysis software that will enable you to follow the 21 CFR Part 11 and data integrity guidelines. We will also be discussing the features and workflow of this new software.
The richness of the image data and the associated features in the IDEAS® Software provides huge benefits for the customer, but also introduces complexity in the data analysis. We address this by providing a tool for automated feature creation using machine learning that enables the creation of new features tailored to sets of image data. We will discuss the details of this tool and provide multiple examples of features generated by the tool to identify populations of interest.
Come to our workshop to hear Neil Bauersfeld discuss the 21 CFR Part 11 enablement tools and Vidya Venkatachalam discuss the machine learning module for high dimensional image data analysis.
 
Bio-Rad Laboratories
2000 Alfred Nobel Drive
Hercules, CA  94547
Phone: 510-408-2158
Email: sonya_sano@bio-rad.com
Web: http://www.bio-rad.com

Presenter:
Chris Trussell, Sr. Scientific Technical Leader/Flow Cytometry Manager

Genomics Institute of Novartis Research Foundation

 
Fast, Automated, High-Throughput Flow Cytometry Using the ZE5 Cell Analyzer

Bio-Rad invites you to learn how high-throughput flow cytometry can be applied to your large-scale screening and validation projects. Learn how the new automation setup, high capacity fluidics tanks, and integrated 96 and 384-well plate sampler for the ZE5 Cell Analyzer can increase your lab’s productivity and throughput, letting you create an automated workcell to run up to 24 hours per day with a single operator.
 
Micromedicine
1440 Main Street, Suite 300
Waltham, MA 02451
Phone: 508-561-6476
Web: www.micromedicine.com

Presenter: Sarah Mickool, Research Engineer, MicroMedicine
 
Automating Cell Separation and Concentration with a High Throughput Microfluidic-based System
 
For the past 50 years, researchers have relied on manual, density gradient centrifugation techniques to meet their cell isolation needs. The field is in desperate need of a new technique that recovers more cells in better health. MicroMedicine is developing an automated, minimally-manipulative, microfluidic-based cell isolation technology for the separation of white blood cells from large volumes of whole blood. This technology will be launched as the Sorterra Cell Isolation System in 2019.
This tutorial will provide an overview of how inertial focusing in microfluidic channels is leveraged to enable efficient and gentle cell separation based on cell size. Data from Sorterra  prototypes show approximately four-log removal of red blood cells and platelets, which is approximately 80x and 1200x better than typical density gradient separation methods, respectively, with approximately 90% white blood cell recovery. When you’re processing whole blood samples, success depends on maximizing the number of cells of interest.  Optimize your cell sorting with SorterraTM to achieve better results and insights.

FlowJo, LLC
385 Williamson Way
Ashland, OR 97520
Phone: 541-201-0022
Email: caitlinf@flowjo.com
Web: http://www.flowjo.com
 
Presenter: Mike Stadnisky, Ph.D.
 
A Hierophant’s Approach to Single Cell Biology: A Brief History of Tomorrow
 
Tarot cards tell the story of humanity’s spiritual evolution from naive to enlightened.  In this tutorial, we will play the role of the Hierophant, and juxtapose traditional views and approaches in single cell biology and rebellious new ways of thinking beyond incrementalism in single cell biology.
 
We will accomplish this task along 4 lines:
(1) We will invert the 20-year plan we have shared widely with customers, and judge the progress of innovations and applications in single cell analysis
(2) We will then take a step back and discuss deeper impediments to understanding, including  the psychology of data analysis and why data sharing seems destined to fail
(3) We will describe new paradigms to enable this including practical tools to address the challenges posed by (1) and (2)
(4) Finally, we will share our work which enables a portable, useful, systems-level understanding of immune cells

ISCT-ISAC JOINT SESSION ON CELL AND IMMUNE THERAPY
13:30 - 15:00 

ISCT-ISAC, A Natural Synergy​
Jonni Moore, Perelman School of Medicine at the University of Pennsylvania, USA

Characterization of Novel Cellular Therapeutics – Safety and Efficacy Evaluations by Flow Cytometry 
Gerhard Bauer, UC Davis Health, USA

Setting Minimum Standards for the Development and Qualification of In-Process and Release QC Assays for Cell & Gene Therapies
Mark Lowdell, Royal Free London, UK

Long-Term Remission of CLL Sustained by Pauciclonal anti-CD19 Chimeric Antigen Receptor T Cells
Jos Melenhorst, University of Pennsylvania, USA

COFFEE BREAK
15:00 - 15:15

FRONTIERS SESSION 2: Highlighting New Cytometry Platforms that Interrogate Diverse Sub-Cellular and Cellular Targets​
15:15 - 16:45

Detection of EV-based Signatures in Prostate Cancer Using Microflow Cytometry and Machine Learning
John Lewis, University of Alberta, Canada

Scalable and Comprehensive Characterization of Antigen-Specific CD8 T Cells Using Multi-Omics Single Cell Analysis​
Stephane Boutet, 10x Genomics, USA

POSTER SESSION 2 PREVIEW: 5-Minute Flash Talks Describing the Highest Scoring Posters
16:45 - 17:00

POSTER SESSION 2
17:00 - 18:00

LE GOÛTER
17:00 - 18:00

WEDNESDAY, 26 JUNE, 2019

CYTO Youth Workshop
By Initiation Only
8:30 - 15:30


AGENDA:
8:30 - 8:40               Welcome/Logistics – Julie Hill/ISAC Representative

8:40 - 8:55               Pre-workshop assessment – Gelo Victoriano Dela Cruz

8:55 – 9:40              Lecture: Introduction to Cytometry – Jennifer Wilshire
                                 "3 Things to Remember"

9:40 – 9:45              Move to Activity Stations

Activity Stations 
9:45 – 10:00            Station 1
10:00 – 10:15          Station 2       
10:15 – 10:30          Station 3
10:30 – 10:45          Break
10:45 – 11:00          Station 4
11:00 – 11:15          Station 5
11:15 – 11:30          Station 6
11:30 – 11:45          Station 7

7 Activity Stations
Macro Marble Flow Cell – Dan Callen
Cytometry Demos/Models – Alexis Conway
Image Cytometry/FlowSight Instrument Demo – Haley Pugsley & Sherree Friend
Cell Sorting – Roxana del Rio-Guerra & Gelo Victoriano Dela Cruz
Flow Jo Data Analysis – Tim Crawford, Emilie Jalbert
Antibody Models – Julie Hill, Reitha Weeks, Donna Prunkard
Instrument Parts – Self-service table – grad students/Jennifer Wilshire

12:00 – 13:00         Lunch in place
                                 Grad student helpers and workshop organizers join student lunch groups
  
13:00 – 14:00         Visit Exhibit Hall

14:00 – 14:45         Lecture: Cytometry Applications - Juan Garcia Vallejo
 
14:45 - 15:30          Elevator speeches, Post-workshop Assessment, Evaluation, Wrap up/Resources

POSTER VIEWING HOURS
8:00 - 16:00

ISAC BUSINESS MEETING
8:00 - 8:30

FRONTIERS SESSION 3: Unique Applications of Cytometry
8:30 - 10:00

High-Content Microscopy for Systems Pharmacology of Cardiac Fibrosis and Regeneration​
Jeffery Saucerman, University of Virginia School of Medicine, USA

Immune Phenotyping of Bronchoalveolar Lavage in Children with Cystic Fibrosis ​
Melanie Neeland, Murdoch Children's Research Institute, Australia

EXHIBITS OPEN
10:00 - 16:00

COFFEE BREAK
10:00 - 10:30

CONCURRENT PARALLEL SESSIONS 15-18
10:30 - 12:00

Parallel Session 15: Cytometry in Infectious Disease and Asthma

Experimental severe malaria is resolved by targeting newly-identified  monocyte subsets with immune modulatory treatment, Paula Niewold, et. al.

Multivariate analyses of high parameter spectral flow cytometry  and  plasma  analyte datasets reveal links between gamma delta T cell subsets and plasma markers of systemic inflammation and/or gut permeability with ART-suppressed HIV infection and healthy aging, Riley Pihl, et. al.
 
Identification of a sepsis-specific neutrophil cellular signature using mass cytometry approach, Aïda Meghraoui-Kheddar, et. al.
 
Immune profiling of asthma patients using FlowSOM, Katrien Quintelier, et. al.

Parallel Session 16: New Sorting News II

Surface Acoustic Wave Microfluidics for Cell Sorting Applications, Kirk Mutafopulos, et. al.
 
Real-Time Image-Based Cell Sorting, Yi Gu, et. al. 
 
Label-free separation of mesenchymal stromal cell subpopulations using acoustophoresis, Franziska Olm, et. al.
 
An engineered bacterial community that enables magnetic sorting of single species to elucidate interbacterial metabolite fluxes in the mammalian intestine, Jakob Zimmermann, et. al.
 
Parallel Session 17: Application of Algorithms

Ditch the ball: automated optimal parameters for T-distributed stochastic neighbor embedding improve visualization and allow analysis of large datasets, Anna Belkina, et. al. 
 
Integration of Mechanistic Immunological Knowledge into a Machine Learning Pipeline Increases Predictive Power of Clinical Studies,  Anthony Culos, et. al.
 
High-dimensional data analysis developed for mass cytometry can successfully be used for spectral flow cytometry data, Laura Ferrer Font, et. al.
 
Immune Reconstitution in Pediatric HIV: Polychromatic Flow Cytometry and Machine Learning Reveal Cell Subsets Not Normalized with Treatment, Aidan Winters, et. al.
 
Parallel Session 18: Biological Insights I

High-dimensional single cell mass cytometry analysis of the murine hematopoietic system reveals distinct alterations induced during immunoaging versus physiological pathogen challenges, Christos Nikolaou, et. al.
 
Chromotyping: Single cell capture of multiplexed chromatin content to reveal functional cell states in human pathobiology, Reema Baskar, et. al.

A metabolic cytometry evaluation of tamoxifen treatment and resistance in breast cancer, David Rodriguez, et. al.

Single-cell RNA-seq reveals a transcriptional program distinguishing short- and long-lived plasma cells, Derek Jones, et. al.

LUNCH
12:00 - 12:30

COMMERCIAL TUTORIALS
12:30 - 13:30


Cytek Biosciences Inc.
46107 Landing Parkway
Fremont, CA 94538
 
Phone: 510-657-0102
Email: greinin@cytekbio.com
Web: https://cytekbio.com/
 
Presenter: Dr. Yacine Kharraz, PhD

Pushing the Limits of Fluorescence in a Fluorochrome Limited World
 
Until recently, developing assays with over 30 colors in the field of fluorescence-based flow cytometry has been aspirational, with many scientists turning to other technologies for their high-parameter applications. No longer. With the addition of the UV laser and a total of 64 fluorescence detectors, Cytek® Aurora now has the power to take highly-multiplexing assays beyond 30 colors without the use of specialty dyes. In this tutorial, Dr. Kharraz will explain how Cytek is able to produce robust, high resolution, reproducible 30+ color data. The talk includes a direct comparison between data generated from the UV-laser Aurora and a Mass Spectroscopy Cytometer. This analysis shows that our unique full spectrum flow cytometer is an equally capable tool for high parametric cell analysis. Join us as we demonstrate the Aurora's full spectrum approach and show that hardware is no longer the limiting factor for achieving ultra-high multiplexed applications with fluorescence-based technology.
 
Sony Biotechnology Inc.
1730 North First St. 
San Jose, CA 95112
Phone: 800-275-5963
Fax: 408-352-4130
Email: jordan.mackinnon@sony.com
Web: http://www.sonybiotechnology.com
 
Presenter: Greg Veltri, PhD
 
Spectral Cytometry – Innovations for Deeper Insights

Sony spectral analyzers achieve a true simplistic workflow through advanced electronics, patented optical technologies and many points of automation.  In addition, the power of spectral analysis technology enhances dim signal detection for better visualization of rare populations, fluorescent proteins and fluorochromes excited by multiple lasers.  Enabling novice and advance flow cytometrist the ability to achieve a greater depth of information, with a more accurate view of their data.  In this presentation Sony will present experiments that demonstrate how innovations in automation and software, improve multicolor analysis, simplify flow and make it more approachable to a wider audience of researchers.
 
Akadeum Life Sciences, LLC
674 S Wagner Rd, Suite 30
Ann Arbor, MI 48103
United States
Phone: 734-707-1233
Web: http://www.akadeum.com

Presenter: Leo J. Ostruszka, Ph.D., Principal Scientist 

Microbubbles: The Next Generation of Cell Isolation

The enrichment and isolation of leukocyte populations is critical to advancing research, and benchtop cell separation has become an important part of many researchers’ daily workflow. In recent years, there has been a growing need for an alternative to immunomagnetic methods. Akadeum is introducing that alternative in our new microbubble technology. This commercial tutorial will present an overview of our microbubble technology and its utility in the following: the isolation of mouse B cells, red blood cell cleanup of flow sorting samples, and an improved method for NK cell isolation.

CONCURRENT PARALLEL SESSIONS 19-22
13:45 - 15:15

Parallel Session 19: Mass Cytometry in Disease Studies

Mass Cytometry Profiling of Cell Signaling Pathways in Active and JAK inhibitor-treated Systemic Lupus Erythematosus Patients, Marie Urbicht, et. al. 
 
CyTOF plus DISCOV-R analysis link the phenotype of rare autoreactive T cells with disease outcome in type 1 diabetes, Alice Wiedeman, et. al. 
 
Glucocorticoid-resistant leukemic B-cells undergo a phenotypic change that increases sensitivity to SRC/ABL inhibition, Jolanda Sarno, et. al. 
 
A year-long immune profile of the systemic response in acute stroke survivors, Amy Tsai, et. al.
 
Parallel Session 20: High Sensitivity Disease Screening

Quantitative single cell detection of mutant IDH1 R132H protein in intraoperative and archival glioma specimens, Justine Sinnaeve, et. al.

Single Antibody  Assessment  of  T-Cell  Clonality  by  Flow Cytometry Rapidly Identifies T-Cell Neoplasms and Monotypic CD8- Positive Subsets of Uncertain Significance, Min Shi, et. al.
 
Single-molecule immunoaggregation assay detects biomarkers in sub-microliter sample, Hao He, et. al. 
 
Potential Application of Image Cytometry in Oral Cancer Screening, Katya Parfenova, et. al.

Parallel Session 21: New Imaging Applications in Hematology/Oncology

“Immuno-flowFISH”: Imaging flow cytometry for the assessment of chromosomal abnormalities in phenotyped chronic lymphocytic leukaemia cells in suspension, Kathy Fuller, et. al.

Label-free cell-based diagnostics: leveraging machine learning to do more with less, Minh Doan, et. al.

Deep insights into tumor biology by a novel cyclic immunofluorescence imaging, Anderas Bosio, et. al.

Development of a deep learning approach to aid leukemia detection without immunophenotyping using imaging flow cytometry, Vidya Venkatachalam, et. al.

Parallel Session 22: Biological Insights II

BCR-ABL-containing leukemic extracellular vesicles contribute to immunosuppression in chronic myeloid leukemia by controlling expression of Foxp3 and biology of regulatory T cells, Julian Swatler, et. al.

Novel chemical platform for the mass cytometry-based multiparametric analysis of  cancer  activome, Katarzyna Groborz, et. al.

Live cell imaging of intracellular calcium and neural transcription factor oscillations: a new approach for understanding GABAergic differentiation, Henning Ulrich, et. al.

Agnostic clustering of RNA-Seq transcripts from single sorted nuclei identifies novel cell  types within maize organs, David Galbraith, et. al.

COFFEE BREAK
15:15 - 15:30

AWARDS CEREMONY
15:30 - 16:30

MEETING TRACK WRAP-UPS

An open discussion highlighting attendees opinions on the most interesting presentations and take-home messages from the meeting.  Discussions will be centered around 6 tracks: Flow Cytometry, Mass Cytometry, Image Cytometry, New Technologies, Informatics, and Biological Insights.
16:30 - 17:15

CLOSING RECEPTION AT SCIENCE WORLD
19:00 - 24:00

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