PRE-CONGRESS COURSES

We are grateful for the support of our course sponsors:

The 5th Annual ISAC Introductory Flow and Image Cytometry Course will be held prior to CYTO 2012, on Friday, June 22nd and Saturday, June 23rd, in Leipzig, Germany.  The courses will include both basic and advanced lectures.  These modules provide an excellent opportunity for attendees to learn from experts in the field.  For a modest cost, they provide an amazing educational value.

Registrants will have the opportunity to select five modules of interest in addition to the first two combined flow and image modules.  Plan to register early, since space is limited and registrations will be accepted on a first come, first served basis!  Click here to register for a course.  Click here to view the course schedule.

Basic Flow & Image Cytometry

The Basic Flow & Image Cytometry modules will be held day Friday, June 22, 2012 and Saturday, June 23, 2012.  It will focus on fundamentals of key technologies used in flow and image cytometry and will illustrate them using state-of-the-art applications.

The course is intended for all students, technicians, and investigators with a desire to refresh and enhance their knowledge of flow and image cytometry, beginning with fundamentals and advancing to more sophisticated applications.

Practical demonstrations with the latest equipment will provide students with an opportunity to incorporate what is learned in the module with direct applications to the machines.

Combined Flow and Image Modules

• Experimental Design and the Applicability of Flow and/or Image Cytometry - Paul J. Robinson
• Fluorescence and Fluorochromes - Sr Faculty: Alan Waggoner and Jr Faculty: Alan Saluk
  

Flow Modules

• Basics of Flow Cytometry - Sr Faculty: Willliam Telford and Jr Faculty: Dayong Jin (Scholar)
  
• Cell Cycle and Proliferation - Sr Faculty: Wim Corver and Jr Faculty: Alfonso Blanco (Scholar)
  
• Apoptosis - Sr Faculty: Michael Ormerod and Jr Faculty: Brian Grimberg (Scholar)
  
• Phenotype Analysis - Sr Faculty: Paresh Jain and Jr Faculty: Tomas Kalina (Scholar)
  
• Stem Cell Analysis - Sr Faculty: Ian Dimmick and Jr Faculty: Kewal Asosingh (Scholar)
  
• Cytokine Analysis - Sr Faculty: Andreas Radbruch and Hyun-Dong Chang and Jr Faculty: Andrea Holme (Scholar)
  
• Standards and Controls - Sr Faculty: Joanne Lannigan and Jr Faculty: Sebastien Coquery
  

Image Modules

• Fundamental Optics and Basic Digital Microscopy - Sr Faculty: Silas Leavesley and Jr Faculty: Rachel Errington and Bartek Rajwa
  
• Basic Image Analysis - Gustavo Rohde
• Multi-Dimensional & Time-Series Microscopy-Tracking Dynamic Events - Rachel Errington
• In-Vivo Imaging - TBD
• Super-Resolution and PALM - Reiner Heinzman
• Quantitative Interactions FRET/FLIP/FCS - Jr Faculty: Tytus Beras (Scholar)
  
• High Content Screening - TBD
• High-Throughput Tissue Cytometry - Sr Faculty: Brian McClintock and Jr Faculty: Donat Alpar (Scholar)
  

Click here for module descriptions.

Click here for the Basic Flow & Image Cytometry Course poster.

• Panel Design, Compensation, Spreading Error
• Considerations for Longitudinal and Multicenter Studies

Visualizing Data

• Visualizing Data and Identifying Populations
• Advanced Visualizations with FlowJo
• Data Analysis in the Cloud
• Troubleshooting Based on Staining Patterns

Reporting Results

• Fundamental Statistics
• MiFlowCyt, MIATA
• Aggregating Data Across Samples

Application-Oriented Data Analysis Issues

• Intracellular Cytokine Staining
• Proliferation (CFSE)/Cell Cycle
• Phosphoantigens

Automated Data Analysis

• Broad Overview: Current Methods and Evaluation Tools
• FlowType
• Frequency Difference Gating and Probability Binning
• Gemstone