WORKSHOPS
SUNDAY, 19 MAY, 2013
15:45 - 17:15
WORKSHOP 1
Navigating the Labyrinth of Regulated Flow Cytometry in Drug Development
Virginia Litwin, Covance, Chantilly, VA, USA
Jennifer Stewart, Flow Contract Site Laboratory, Krikland, WA, USA
Jennifer Olsen, Covance, Chantilly, VA, USA
Joel Puchalski, Covance, Chantille, VA, USA
Cherie Green, Amgen, Washington, DC, USA
Christopher Wiwi, Celgene, Summit, NJ, USA
Attendees at this workshop will learn how flow cytometry is used in regulated environments and have the opportunity to share their experiences and challenges in this arena. In addition, participants will learn how adhering to the regulations can increase the quality of their data.
WORKSHOP 2
The Delivery of Image-Based Cytometry Education via CYTO U
John Nolan, La Jolla Bioengineereing Institute, San Diego, CA, USA
Bartek Rajwa, Purdue University, West Lafayette, IN, USA
Rachel Errington, Cardiff University, Cardiff, United Kingdom
Gyorgy Vereb,University of Debrecen, Debrecen, Hungary
Stephen Lockett, SAIC, Frederick, MD, USA
Anil Parwani, University of Pittsburgh, Pittsburgh, PA, USA
Gustavo K. Rohde,
Carnegie Mellon University, Pittsburgh, PA, USA
At the workshop we will present and describe the goals of ISAC's/CYTO University program. We will specifically detail aspects for the current plans for image-based cytometry online education. Thus the workshop includes an overview of current microscopic imaging modalities and techniques, a review of current training programs in microscopy (live and online), as well as a review of other online resources, amongst other topics. The workshop will provide an opportunity for all interested parties to participate in discussions to shape the curriculum and methods for image cytometry education.
WORKSHOP 3
Biosafety: Biosafety Policy Meets Real Life Scenarios
Kevin L. Holmes, NIH, NIAID, Bethesda, MD, USA
Stephen Perfetto, Vaccine Research Center NIH, Bethesda, MD, USA
Since the first ISAC guidelines published in 1997, the ISAC has provided the research community with valuable guidance to the biosafety of cell sorting. In 2007, these guidelines were updated and upgraded to standards to best reflect the importance of these procedures and policies in laboratories around the world.
This workshop will outline the NIH Policy and also present sample questions/scenarios of real-life situations encountered by cell sorter operators and guidelines to determine the appropriate biosafety procedures and practices.
WORKSHOP 4
Quantitative Cytometry - Calibration and Standardization
Lili Wang, NIST, Gaithersburg, MD, USA
Robert Hoffman, BD Biosciences, San Jose, CA, USA
Long term and cross laboratory collaborative studies require appropriate calibration and standardization protocols. This workshop will concentrate on standards and reference materials for both instrument calibration and assay calibration/standardization. The workshop will summarize current state of standards and reference materials, as well the gaps to be filled and needs remaining for cytometry standardization. The workshop will have a series of presentations followed by discussion, with the aim of defining goals for a collaborative study with which assay calibration and standardization can be demonstrated through the use of standards, reference controls, and a validated assay procedure.
WORKSHOP 5
Functional Analysis of Mitochondria and Transporters
George Babcock, University of Cincinnati, Cincinnati, OH, USA
Padma Narayanan, Amgen, Seattle, WA, USA
Early prediction of mitochondrial perturbation (MP) and impairment of hepatocellular bile acid transport (HBAT) by novel drug candidates is becoming a critical feature in drug development. In this workshop we will describe approaches for screening and diagnosing drug candidates for their potential to cause MP and HBAT utilizing various fluorescence based platforms. We discuss the strength and limitations of various screens and provide recommendations of where to position these assays during the drug commercialization process.
MONDAY, 20 MAY, 2013
15:45 - 17:15
WORKSHOP 6
Integrating Standards at the Interface of Flow and Image Cytometry
Hans Minderman, Roswell Park Cancer Institute, Buffalo, NY, USA
Andrew Filby, Cancer Research UK, London, United Kingdom
Anne Plant, National Institute of Standards and Technology, Gaithersburg, MD, USA
Michael Halter, National Institute of Standards and Technology, Gaithersburg, MD, USA
Padma Narayanan, Amgen, Washington DC, USA
Amanda White, Cincinnati Children's Hospital,
Cincinnati, OH, USA
In recent years, flow cytometry has benefitted greatly from the recognized MIFlowCyt standards that cover key areas such as quality control, instrument performance, experimental set up and data reporting. With the emergence of image cytometry platforms that preserve the high event throughput of flow cytometry while delivering high content, quantitative imaging, this is an opportune time to open a cross-platform multi-discipline dialogue as to how MIFlowCyt and Imaging standards could be applied to image cytometry. While there are many aspects to this topic that deserve in-depth discussion and both the time and dedication outside the time constraints of this workshop, we will aim to initiate discussion and dialogue pertaining to a need to standardize data acquisition, analysis and reporting for IC in a manner akin to MIFlowCyt.
WORKSHOP 7
Writing, Publishing and Reviewing: Advice and Tips from Cytometry A
Attila Tarnok, University Leipzig, Leipzig, Germany
Ryan Brinkman, British Columbia Cancer Agency, Vancouver, Canada
Vera Donnenberg, University of Pittsburgh, Pittsburgh, PA, USA
Henning Ulrich, Univeristy of Sao Paulo, Sao Paulo, Brazil
Susan Vice, Wiley, Hoboken, NJ, USA
Scientific journals require certain quality standards from manuscripts to be acceptable for further reviewing and publication. There are some very common reasons why a paper gets reviewed and accepted or rejected. This workshop aims to highlight all major aspects of manuscript writing, submission and communication with the reviewers, points out what can (and very often does) go wrong and how to do it right in order to improve your chances to get your paper published. Special emphasis will be taken to focus on the needs for a biomedical and technical oriented journal such as Cytometry Part A including MIFlowCyt, FlowRepository and OMIP. Experts from the Cytometry Part A editorial board and from the publisher Wiley-Blackwell will contribute. Winner of the best paper award of Cytometry Part A will briefly present the winning work. Authors may bring their manuscript drafts for discussion with the editors after the workshop.
WORKSHOP 8
High Throughput Flow Cytometry in Pharma
Bruce Edwards, Univeristy of New Mexico, Albuquerque, NM, USA
Rob Jepras, GlaxoSmithKline, Middlesex, United Kingdom
In this workshop we are soliciting input from industrial and academic laboratories that have experience with high capacity flow cytometry on topics such as: assay development and validation, techniques and tricks for efficient sample and plate preparation, perspectives on the role of high capacity flow cytometry in high content screening and systems biology, recent developments in hardware and software, favorite auxiliary equipment excluding the flow cytometer/sampling platform, and future needs and trends.
WORKSHOP 9
Mesenchymal Stem Cell Identification and Characterization
Vera Donnenberg, University of Pittsburgh, Pittsburgh, PA, USA
Keith Wonnacott, National Institutes of Health, Bethesda, MD, USA
Albert Donnenberg, University of Pittsburgh, Pittsburgh, PA, USA
Henning Ulrich, University of Sao Paulo, Sao Paulo, Brazil
Anne Plant, National Institute of Standards and Technology, Gaithersburg, MD, USA
Special emphasis will be taken to focus on the needs for the development and availability of validated, robust, automatable, GMP-compatible methods for the identification and isolation of human MSC. Experts from the Food and Drug Administration Cellular Therapies Branch were invited to participate, the National Institute of Standards and Technology the cytometry community and cellular products manufacturing and testing laboratories will be available for a panel discussion.
The goal of this workshop is to outline a set of procedures central to assay qualification, validation and quality assessment, as well as training and qualification of personnel.
WORKSHOP 10
Malaria Workshop
Howard Shapiro, Howard Shapiro, M.D., P.C., West Newton, MA, USA
Grace Chojnowski, Queensland Institute of Medical Research, Brisbane, Australia
Provide a forum for interaction to enable development current and new applications in cytometry for analysis of malaria in research and clinical diagnosis. Core managers, malaria researchers, and clinicians would all greatly benefit from such a workshop. The cytometry community has risen to the challenge of making CD4 counts for HIV-infected individuals easier and more accessible at the point of care in both resource-poor and affluent countries; what we have learned from that effort should enable us to have at least an equivalent impact in the fight against malaria.
TUESDAY, 21 MAY, 2013
15:45 - 17:15
WORKSHOP 11
Design and Application of Receptor Occupancy Assays used to Measure Pharmacodynamic Response to Treatment with Biologic Therapies
Virginia Litwin, Covance, Indianapolis, IN, USA
Cherie Green, Amgen, Thousand Oaks, CA, USA
Marna Wiliams, Genentech, San Francisco, CA, USA
David Wunderlich, Pfizer, Washington, DC, USA
Meina Liang, MedImmune, Gaithersburg, MD, USA
John Ferbas, Amgen, Thousan Oaks, CA, USA
This session will focus on RO assays including 1) overview of what a flow cytometric RO assay is and the information that can and cannot be obtained with this type of assay, 2) case studies of assay development and validation, including technical challenges, 3) modeling and correlation with PK and difficulties encountered for data interpretation. Unique aspects of RO assays will be discussed (eg, selection of competitive and non-competitive antibodies, impact of increased soluble receptor, receptor internalization, and receptor mediated clearance).
WORKSHOP 12
Microvesicle Analysis
Nancy Fisher, University of North Carolina, Chapel Hill, NC, USA
Joanne Lannigan, University of Virginia, Charlottesville, VA, USA
Small membrane vesicles released from cells (aka microvesicles, exosomes, ectosomes, microparticles) are attracting increased interest as diagnostic and therapeutic targets. Flow cytometry is an attractive method for the analysis and separation of these biological particles, but their small size and dim signals challenge the sensitivity of commercial instruments. A consequence of this is that many aspects of instrument set up, calibration, and experimental design that are trivial for the measurement of large cells become critically important for accurate microvesicle analysis. This also helps define targets for improvements in flow cytometry and other measurement technologies that are emerging as complementary techniques for classification and characterization of microvesicles. This workshop will address some of these issues through short presentations and discussion.
WORKSHOP 13
Image Cytometry: Spectral Imaging and Tissue Cytometry
Silas Leavesley, University of South Alabama, Mobile, AL, USA
James Mansfield, PerkinElmer, Waltham, MA, USA
Spectral imaging is a set of techniques that offer significant capabilities for image cytometry, especially for multiparameter and multiplexed analysis. Tissue cytometry represents a subset of image cytometry that has significant implications for diagnostic pathology and personalized medicine. There are numerous potential advantages for applying spectral imaging approaches for tissue cytometry, including addressing autofluorescence and other background issues as well as enabling higher levels of multiplexing. In this workshop we will review the available and emerging capabilities for spectral imaging, the state of the art in tissue cytometry, present novel applications such as the enumeration and phenotyping of subsets of immune cells and discuss the challenges and opportunities in these areas.
WORKSHOP 14
Career Development: Cytometry STILL Needs You, but do You Need Cytometry?
Rachael Walker, Babraham Institute, Cambridge, United Kingdom
Andrew Filby, Cancer Research UK, London, United Kingdom
We aim in this workshop to discuss how to develop your career in the various roles that use Cytometry, whether it be working in a core lab, Pharma, being a student using Cytometry or working for a commercial company. There will be brief talks on the various cytometry-related career choices, which we hope will turn into a lively debate on the demands of each career choice and how to progress in each field. We will share experiences of working in industry, core-facilities and jobs associated with cytometry. The workshop aims to be interactive and we will be open to discuss any issue that arise such as how to set up a lab from scratch, running a core as a one (wo)man show, what you need to know to work in a core facility, what should be put on your CV to get you the dream job and how to develop your role.
WORKSHOP 15
Trends in Cytometry Instrumentation
Steven W. Graves, University of New Mexico, Albuquerque, NM, USA
Giacomo Vacca, Kinetic River Corporation, San Jose, CA, USA
This workshop will present the latest technology advancements in Cytometry Instrumentation. We will have specific focus areas on the challenges and opportunities for chip-based cytometry, new detection modalities, and implementation of force based particle micromanipulation approaches in flow cytometry. New work in these areas will be presented in short oral presentations. We will also discuss future trends and challenges in these areas.